Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Chitin and chitosan preparation from shrimp shells using optimized enzymatic deproteinization
Process Biochemistry, Volume 47, No. 12, Year 2012
Notification
URL copied to clipboard!
Description
Different crude microbial proteases were applied for chitin extraction from shrimp shells. A Box-Behnken design with three variables and three levels was applied in order to approach the prediction of optimal enzyme/substrate ratio, temperature and incubation time on the deproteinization degree with Bacillus mojavensis A21 crude protease. These optimal conditions were: an enzyme/substrate ratio of 7.75 U/mg, a temperature of 60 °C and an incubation time of 6 h allowing to predict 94 ± 4% deproteinization. Experimentally, in these optimized conditions, a deproteinization degree of 88 ± 5% was obtained in good agreement with the prediction and larger than values generally given in literature. The deproteinized shells were then demineralized to obtain chitin which was converted to chitosan by deacetylation and its antibacterial activity against different bacteria was investigated. Results showed that chitosan dissolved at 50 mg/ml markedly inhibited the growth of most Gram-negative and Gram-positive bacteria tested. © 2012 Elsevier Ltd.
Authors & Co-Authors
Younes, Islem
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Ghorbel-Bellaaj, Olfa
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Nasri, Rim
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Châabouni, Moncef M.
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Rinaudo, Marguerite
France, Grenoble
European Synchrotron Radiation Facility
France, Grenoble
Centre de Recherches Sur Les Macromolécules Végétales
Nasri, Moncef
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Statistics
Citations: 161
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.procbio.2012.07.017
ISSN:
13595113