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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes
Molecular Plant Pathology, Volume 13, No. 8, Year 2012
Notification
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Description
Plant parasitic nematodes impose a severe constraint on plantain and banana productivity; however, the sterile nature of many cultivars precludes conventional breeding for resistance. Transgenic plantain cv. Gonja manjaya (Musa AAB) plants, expressing a maize cystatin that inhibits nematode digestive cysteine proteinases and a synthetic peptide that disrupts nematode chemoreception, were assessed for their ability to resist nematode infection. Lines were generated that expressed each gene singly or both together in a stacked defence. Nematode challenge with a single species or a mixed population identified 10 lines with significant resistance. The best level of resistance achieved against the major pest species Radopholus similis was 84% ± 8% for the cystatin, 66% ± 14% for the peptide and 70% ± 6% for the dual defence. In the mixed population, trial resistance was also demonstrated to Helicotylenchus multicinctus. A fluorescently labelled form of the chemodisruptive peptide underwent retrograde transport along certain sensory dendrites of R. similis as required to disrupt chemoreception. The peptide was degraded after 30min in simulated intestinal fluid or boiling water and after 1h in nonsterile soil. Insilico sequence analysis suggests that the peptide is not a mammalian antigen. This work establishes the mode of action of a novel nematode defence, develops the evidence for its safe and effective deployment against multiple nematode species and identifies transgenic plantain lines with a high level of resistance for a proposed field trial. © 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD.
Authors & Co-Authors
Roderick, Hugh
United Kingdom, Leeds
University of Leeds
Tripathi, Leena
Uganda, Kampala
International Institute of Tropical Agriculture Uganda
Babirye, Annet
Uganda, Kampala
International Institute of Tropical Agriculture Uganda
Wang, Dong
United Kingdom, Leeds
University of Leeds
China, Beijing
Chinese Academy of Sciences
Tripathi, Jaindra Nath
Uganda, Kampala
International Institute of Tropical Agriculture Uganda
Urwin, Peter E.
United Kingdom, Leeds
University of Leeds
Atkinson, H. J.
United Kingdom, Leeds
University of Leeds
Statistics
Citations: 60
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1111/j.1364-3703.2012.00792.x
ISSN:
14646722
e-ISSN:
13643703
Research Areas
Environmental
Genetics And Genomics
Study Design
Cross Sectional Study