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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Trypanosoma evansi: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates
Experimental Parasitology, Volume 114, No. 3, Year 2006
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Description
We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses. AFLP accessed many more polymorphisms than RAPD. Cluster analysis of the AFLP data showed that 12 T.evansi isolates were very similar ('type A') whereas 2 isolates differed substantially ('type B'). Type A isolates have been generally regarded as genetically identical but AFLP analysis was able to identify multiple differences between them and split the type A T. evansi isolates into two distinct clades. © 2006 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Masiga, Daniel K.
United Kingdom, Glasgow
Ibls
Kenya, Nairobi
Kenyatta University
Ndung'u, Kariuki
Kenya, Nairobi
Kenya Agricultural Research Institute
Tweedie, Alison
United Kingdom, Glasgow
University of Glasgow
Tait, Andrew B.
United Kingdom, Glasgow
University of Glasgow
Turner, Charles Michael R.
United Kingdom, Glasgow
Ibls
United Kingdom, Glasgow
University of Glasgow
Statistics
Citations: 34
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/j.exppara.2006.03.002
ISSN:
00144894
e-ISSN:
10902449
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study