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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
HIV-1 Pr55Gag binds genomic and spliced RNAs with different affinity and stoichiometry
RNA Biology, Volume 14, No. 1, Year 2017
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Description
The HIV-1 Pr55Gag precursor specifically selects genomic RNA (gRNA) from a large variety of cellular and spliced viral RNAs (svRNAs), however the molecular mechanisms of this selective recognition remains poorly understood. To gain better understanding of this process, we analyzed the interactions between Pr55Gag and a large panel of viral RNA (vRNA) fragments encompassing the main packaging signal (Psi) and its flanking regions by fluorescence spectroscopy. We showed that the gRNA harbors a high affinity binding site which is absent from svRNA species, suggesting that this site might be crucial for selecting the HIV-1 genome. Our stoichiometry analysis of protein/RNA complexes revealed that few copies of Pr55Gag specifically associate with the 5′ region of the gRNA. Besides, we found that gRNA dimerization significantly impacts Pr55Gag binding, and we confirmed that the internal loop of stem-loop 1 (SL1) in Psi is crucial for specific interaction with Pr55Gag. Our analysis of gRNA fragments of different length supports the existence of a long-range tertiary interaction involving sequences upstream and downstream of the Psi region. This long-range interaction might promote optimal exposure of SL1 for efficient Pr55Gag recognition. Altogether, our results shed light on the molecular mechanisms allowing the specific selection of gRNA by Pr55Gag among a variety of svRNAs, all harboring SL1 in their first common exon. © 2017 Taylor & Francis Group, LLC.
Authors & Co-Authors
Abd El–Wahab, Ekram W.
France, Paris
Cnrs Centre National de la Recherche Scientifique
Mak, Johnson
Australia, Melbourne
Burnet Institute
Australia, Clayton
Monash University
Australia, Geelong
Deakin University
Australia, Canberra
Commonwealth Scientific and Industrial Research Organisation
Marquet, Roland
France, Paris
Cnrs Centre National de la Recherche Scientifique
Statistics
Citations: 42
Authors: 3
Affiliations: 5
Identifiers
Doi:
10.1080/15476286.2016.1256533
ISSN:
15476286
Research Areas
Genetics And Genomics
Infectious Diseases