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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Effects of H
2
O
2
exposure on human sperm motility parameters, reactive oxygen species levels and nitric oxide levels
Andrologia, Volume 42, No. 3, Year 2010
Notification
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Description
Research has revealed that reactive oxygen species (ROS) negatively affect sperm function, both in vivo and in vitro. Sperm preparation techniques for assisted reproductive technologies (ART) are potential causes for additional ROS production. This study aimed to correlate the concentration of exogenous H 2O2 with sperm motility parameters and intracellular ROS and nitric oxide (NO) levels to reiterate the importance of minimising ROS levels in ART. Human spermatozoa from 10 donors were incubated and exposed to different exogenous H2O2 concentrations (0, 2.5, 7.5 and 15 μm). Subsequently, motility was determined using computer-aided semen analysis, while ROS (2,7-dichlorofluorescin diacetate) and NO (diaminofluorescein-2/diacetate) were analysed using fluorescence-activated cell sorting. Results showed that H2O2 did affect the sperm parameters. Exogenous H2O2 was detrimental to motility and resulted in a significant increase in overall ROS and NO levels. A significant increase in static cells was seen as well. It is important to elucidate the mechanisms between intracellular ROS levels with sperm motility parameters. While this experiment demonstrated a need to reduce exogenous ROS levels during ART, it did not illustrate the cause and effect relationship of intracellular ROS and NO levels with sperm motility. Further research needs to be conducted to define a pathological level of ROS. © 2010 Blackwell Verlag GmbH.
Authors & Co-Authors
Du Plessis, Stefan Stephanus
South Africa, Stellenbosch
Stellenbosch University
McAllister, David A.
South Africa, Stellenbosch
Stellenbosch University
Luu, Allan M.
United States, Cleveland
Cleveland Clinic Foundation
Savia, J.
United States, Cleveland
Cleveland Clinic Foundation
Agarwal, Ashok K.
United States, Cleveland
Cleveland Clinic Foundation
Lampiao, Fanuel
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 100
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1111/j.1439-0272.2009.00980.x
ISSN:
03034569
e-ISSN:
14390272
Research Areas
Sexual And Reproductive Health
Study Approach
Quantitative