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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Hydroxylated near-infrared BODIPY fluorophores as intracellular pH sensors
Analyst, Volume 139, No. 19, Year 2014
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Description
In this study, a series of new, highly sensitive BF2-chelated tetraarylazadipyrromethane dyes are synthesized and analyzed to be suitable as on/off photo-induced electron transfer modulated fluorescent sensors for determination of intracellular pH. The ethanolic solutions of the new indicators feature absorption maxima in the range of 696–– 700 nm and a fluorescence emission maximum at 720 nm. Molar absorptivity and fluorescence quantum yield data were determined for the studied set of aza-BODIPY indicators. These indicators have high molar absorption coefficients of 80 000 M−1 cm−1 and quantum yields (up to 18%). Corresponding pKa values of indicators are determined from absorbance and fluorescence measurements and range from 9.1 to 10.8, depending on the selective positioning of electron-donating functionalities. The excellent photostability of the aza-BODIPY indicators makes them particularly suitable for long duration measurements. The in vitro cellular staining of living tissues in PC3 cells based on the isosbestic point at pH 7.8 and pH 9.3 has been employed which shows an increase in fluorescence intensity at 800 nm with increase in pH for certain compounds and fluorescence intensity decreases at 700 nm. Therefore, the new indicators are suitable for exploitation and adaptation in a diverse range of analytical applications. © 2014 the Partner Organisations.
Authors & Co-Authors
Salim, Mohamed M.
United States, Atlanta
Georgia State University
Egypt, Mansoura
Faculty of Pharmacy
Owens, Eric A.
United States, Atlanta
Georgia State University
United States, Atlanta
Center for Diagnostics and Therapeutics
Gao, Tielong
United States, Atlanta
Georgia State University
Lee, Jeong Heon
United States, Boston
Harvard Medical School
Hyun, Hoon
United States, Boston
Harvard Medical School
Choi, Hak Soo
United States, Boston
Harvard Medical School
Henary, Maged Mories
United States, Atlanta
Georgia State University
United States, Atlanta
Center for Diagnostics and Therapeutics
United States, Atlanta
Center for Biotechnology and Drug Design
Statistics
Citations: 28
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1039/c4an01104j
ISSN:
00032654
e-ISSN:
13645528
Research Areas
Environmental