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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved Human Peripheral Blood Mononuclear Cells
Journal of Immunological Methods, Volume 409, Year 2014
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Description
The Center for HIV/AIDS Vaccine Immunology (CHAVI) consortium was established to determine the host and virus factors associated with HIV transmission, infection and containment of virus replication, with the goal of advancing the development of an HIV protective vaccine. Studies to meet this goal required the use of cryopreserved Peripheral Blood Mononuclear Cell (PBMC) specimens, and therefore it was imperative that a quality assurance (QA) oversight program be developed to monitor PBMC samples obtained from study participants at multiple international sites. Nine site-affiliated laboratories in Africa and the USA collected and processed PBMCs, and cryopreserved PBMC were shipped to CHAVI repositories in Africa and the USA for long-term storage. A three-stage program was designed, based on Good Clinical Laboratory Practices (GCLP), to monitor PBMC integrity at each step of this process. The first stage evaluated the integrity of fresh PBMCs for initial viability, overall yield, and processing time at the site-affiliated laboratories (Stage 1); for the second stage, the repositories determined post-thaw viability and cell recovery of cryopreserved PBMC, received from the site-affiliated laboratories (Stage 2); the third stage assessed the long-term specimen storage at each repository (Stage 3). Overall, the CHAVI PBMC QA oversight program results highlight the relative importance of each of these stages to the ultimate goal of preserving specimen integrity from peripheral blood collection to long-term repository storage. © 2014 Elsevier B.V.
Authors & Co-Authors
Sarzotti-Kelsoe, Marcella
United States, Durham
Duke University Medical Center
Needham, Leila K.
United States, Durham
Duke University Medical Center
Rountree, Wes
United States, Durham
Duke University Medical Center
Bainbridge, John
United States, Durham
Duke University Medical Center
Gray, Clive M.
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Cape Town
Faculty of Health Sciences
Fiscus, Susan A.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Ferrari, Guido
United States, Durham
Duke University Medical Center
Stevens, Wendy Susan
South Africa, Johannesburg
National Health Laboratory Service
South Africa, Johannesburg
Clinical Laboratory Services
Stager, Susan L.
United States, Durham
Duke University Medical Center
Binz, Whitney
United States, Durham
Duke University Medical Center
Louzao, Raul
United States, Durham
Duke University Medical Center
Long, Kristy O.
United States, Durham
Duke University Medical Center
Mokgotho, Pauline
South Africa, Johannesburg
National Institute for Communicable Diseases
Moodley, Niranjini
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
Clinical Laboratory Services
Mackay, Melanie
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
Clinical Laboratory Services
Kerkau, Melissa
United States, Chapel Hill
The University of North Carolina at Chapel Hill
McMillion, Takesha
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Kirchherr, Jennifer L.
United States, Durham
Duke University Medical Center
Soderberg, Kelly A.
United States, Durham
Duke University Medical Center
Haynes, Barton F.
United States, Durham
Duke University Medical Center
Denny, Thomas N.
United States, Durham
Duke University Medical Center
Statistics
Citations: 21
Authors: 21
Affiliations: 6
Identifiers
Doi:
10.1016/j.jim.2014.05.013
ISSN:
00221759
e-ISSN:
18727905
Research Areas
Infectious Diseases