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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Antioxidant properties of chlorophyll-enriched and chlorophyll-depleted polyphenolic fractions from leaves of vernonia amygdalina and gongronema latifolium
Food Research International, Volume 44, No. 8, Year 2011
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Description
This study investigated the optimum polyphenol extraction conditions for two Nigerian green leafy vegetables, Vernonia amygdalina (VA) and Gongronema latifolium (GL) that are widely used for food and medicinal purposes. Seven different solvents were used to extract the total phenolic contents of the leaves; 100% acetone, 80% acetone, 50% acetone, 100% ethanol, 70% ethanol, 100% methanol and 70% methanol and their total phenolic contents were determined. The results showed that the 80% acetone was significantly (p. <. 0.05) the most effective for polyphenol extraction from the leaves and generally the acetone-containing solvents were more effective than alcohol-containing solvents. The 80% acetone extract was fractionated on a silicic acid-packed column to give two main fractions: acetone eluted chlorophyll-enriched (flow-through) and ethanol eluted chlorophyll-depleted (column-bound). The ethanol fractions of VA and GL were significantly better (p. <. 0.05) DPPH radical scavengers than the acetone fractions. In contrast, the acetone fraction was a significantly better (p. <. 0.05) scavenger of hydroxyl radicals when compared to the ethanol fraction. Both acetone and ethanol fractions significantly (p. <. 0.05) and dose-dependently inhibited linoleic acid oxidation, though duration of inhibition was less at lower concentrations (10 and 25. μg/ml) than at the highest concentration (50. μg/ml). © 2011 Elsevier Ltd.
Authors & Co-Authors
Fasakin, Comfort F.
Nigeria, Akure
Federal University of Technology, Akure
Canada, Winnipeg
University of Manitoba
Udenigwe, Chibuike C.
Canada, Winnipeg
University of Manitoba
Aluko, Rotimi Emmanuel
Canada, Winnipeg
University of Manitoba
Canada, Winnipeg
Richardson Centre for Functional Foods and Nutraceuticals
Statistics
Citations: 45
Authors: 3
Affiliations: 3
Identifiers
Doi:
10.1016/j.foodres.2010.12.019
ISSN:
09639969
Research Areas
Food Security
Substance Abuse