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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species
International Journal of Systematic and Evolutionary Microbiology, Volume 61, No. 6, Year 2011
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Description
'Mycobacterium sherrisii' is an undescribed species that appears to be emerging, in particular, among HIV-positive patients originating from Africa. To describe 'M. sherrisii', to ensure that the species name is validly published and to define its phylogenetic position, we collected 11 of these strains reported in five previous studies, and subjected them to biochemical identification, cell-wall mycolic acid analysis and sequencing of multiple housekeeping genes. The bacteria formed smooth and generally non-chromogenic colonies after 2-3 weeks of subculture at 24-37 °C; photochromogenic and scotochromogenic pigmentation were exhibited by three and two strains, respectively. The strains were positive for the heat-stable catalase test, but negative in tests for hydrolysis of Tween 80, nitrate reduction, β-glucosidase and 3-day arylsulfatase. Mycolic acid patterns, obtained by HPLC, resembled a trimodal profile similar to those of type strains of Mycobacterium simiae, Mycobacterium lentiflavum, Mycobacterium triplex and Mycobacterium genavense. The 16S rRNA gene sequences of the 11 strains differed by 4 bp (99.7 % similarity) from that of the type strain of the closest related species, M. simiae ATCC 25275 T. Levels of internal transcribed spacer (ITS) and partial hsp65 and rpoB gene sequence similarity between the two taxa were 95.8 % (271/283 bp), 97.5 % (391/401 bp) and 95.2 % (700/735 bp), respectively. On the basis of these results, we propose the formal recognition of Mycobacterium sherrisii sp. nov. The type strain is 4773 T (= ATCC BAA-832 T = DSM 45441 T). © 2011 IUMS.
Authors & Co-Authors
Van Ingen, Jakko
Netherlands, Nijmegen
Radboud University Medical Center
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Tortoli, Enrico
Italy, Florence
Azienda Ospedaliera Careggi
Selvarangan, Rangaraj
United States, Kansas City
Children's Mercy Hospitals and Clinics
Coyle, Marie B.
United States, Seattle
University of Washington
Crump, John A.
United States, Durham
Duke University Medical Center
United States, Durham
Duke University
Tanzania, Moshi
Kilimanjaro Christian Medical Centre
Tanzania, Moshi
Kilimanjaro Christian Medical University College
Morrissey, Anne B.
United States, Durham
Duke University Medical Center
Dekhuijzen, Pieter Nicolaas Richard
Netherlands, Nijmegen
Radboud University Medical Center
Boeree, Martin Johan
Netherlands, Nijmegen
Radboud University Medical Center
van Soolingen, Dick
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Statistics
Citations: 30
Authors: 9
Affiliations: 9
Identifiers
Doi:
10.1099/ijs.0.024752-0
ISSN:
14665026
Research Areas
Genetics And Genomics
Infectious Diseases