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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Diversity arrays technology (DArT) markers in apple for genetic linkage maps
Molecular Breeding, Volume 29, No. 3, Year 2012
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Description
Diversity Arrays Technology (DArT) provides a high-throughput whole-genome genotyping platform for the detection and scoring of hundreds of polymorphic loci without any need for prior sequence information. The work presented here details the development and performance of a DArT genotyping array for apple. This is the first paper on DArT in horticultural trees. Genetic mapping of DArT markers in two mapping populations and their integration with other marker types showed that DArT is a powerful high-throughput method for obtaining accurate and reproducible marker data, despite the low cost per data point. This method appears to be suitable for aligning the genetic maps of different segregating populations. The standard complexity reduction method, based on the methylation-sensitive PstI restriction enzyme, resulted in a high frequency of markers, although there was 52-54% redundancy due to the repeated sampling of highly similar sequences. Sequencing of the marker clones showed that they are significantly enriched for low-copy, genic regions. The genome coverage using the standard method was 55-76%. For improved genome coverage, an alternative complexity reduction method was examined, which resulted in less redundancy and additional segregating markers. The DArT markers proved to be of high quality and were very suitable for genetic mapping at low cost for the apple, providing moderate genome coverage. © 2011 The Author(s).
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3285764/bin/11032_2011_9579_MOESM1_ESM.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3285764/bin/11032_2011_9579_MOESM2_ESM.docx
Authors & Co-Authors
Schouten, Henk J.
Unknown Affiliation
Van De Weg, W. E.
Unknown Affiliation
Carling, Jason
Unknown Affiliation
Khan, Sabaz Ali
Unknown Affiliation
McKay, Steven J.
Unknown Affiliation
van Kaauwen, Martijn
Unknown Affiliation
Wittenberg, Alexander H.J.
Unknown Affiliation
Koehorst-van Putten, Herma J.J.
Unknown Affiliation
Noordijk, Yolanda
Unknown Affiliation
Gao, Zhongshan
Unknown Affiliation
Rees, David Jasper Gilbert
Unknown Affiliation
van Dyk, Maria M.
Unknown Affiliation
Jaccoud, Damian
Unknown Affiliation
Considine, Michael J.
Unknown Affiliation
Kilian, Andrzej
Unknown Affiliation
Statistics
Citations: 49
Authors: 15
Affiliations: 10
Identifiers
Doi:
10.1007/s11032-011-9579-5
ISSN:
13803743
Research Areas
Genetics And Genomics
Health System And Policy