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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Co-occurrence and distribution of East (L1014S) and West (L1014F) African knock-down resistance in Anopheles gambiae sensu lato population of Tanzania
Tropical Medicine and International Health, Volume 19, No. 3, Year 2014
Notification
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Description
Objective: Insecticide resistance molecular markers can provide sensitive indicators of resistance development in Anopheles vector populations. Assaying these makers is of paramount importance in the resistance monitoring programme. We investigated the presence and distribution of knock-down resistance (kdr) mutations in Anopheles gambiae s.l. in Tanzania. Methods: Indoor-resting Anopheles mosquitoes were collected from 10 sites and tested for insecticide resistance using the standard WHO protocol. Polymerase chain reaction-based molecular diagnostics were used to genotype mosquitoes and detect kdr mutations. Results: The An. gambiae tested were resistance to lambdacyhalothrin in Muheza, Arumeru and Muleba. Out of 350 An. gambiae s.l. genotyped, 35% were An. gambiae s.s. and 65% An. arabiensis. L1014S and L1014F mutations were detected in both An. gambiae s.s. and An. arabiensis. L1014S point mutation was found at the allelic frequency of 4-33%, while L1014F was at the allelic frequency 6-41%. The L1014S mutation was much associated with An. gambiae s.s. (χ2 = 23.41; P < 0.0001) and L1014F associated with An. arabiensis (χ2 = 11.21; P = 0.0008). The occurrence of the L1014S allele was significantly associated with lambdacyhalothrin resistance mosquitoes (Fisher exact P < 0.001). Conclusion: The observed co-occurrence of L1014S and L1014F mutations coupled with reports of insecticide resistance in the country suggest that pyrethroid resistance is becoming a widespread phenomenon among our malaria vector populations. The presence of L1014F mutation in this East African mosquito population indicates the spreading of this gene across Africa. The potential operational implications of these findings on malaria control need further exploration. © 2014 The Authors.
Authors & Co-Authors
Kabula, Bilali I.
Tanzania, Tanga
National Institute for Medical Research Tanga
Tanzania, Moshi
Kilimanjaro Christian Medical University College
Kisinza, William N.W.
Tanzania, Tanga
National Institute for Medical Research Tanga
Tungu, Patrick Kjia
Tanzania, Tanga
National Institute for Medical Research Tanga
Ndege, Chacha M.
Tanzania, Tanga
National Institute for Medical Research Tanga
Batengana, Bernard Malongo
Tanzania, Tanga
National Institute for Medical Research Tanga
Kollo, Douglas
Tanzania, Tanga
National Institute for Medical Research Tanga
Malima, Robert C.
Tanzania, Tanga
National Institute for Medical Research Tanga
Kafuko, Jessica
Tanzania, Dar es Salaam
U.s. President’s Malaria Initiative
Mohamed, Mahdi
United States, Research Triangle Park
Rti International
Magesa, Stephen Masingili
Tanzania, Tanga
National Institute for Medical Research Tanga
United States, Research Triangle Park
Rti International
Statistics
Citations: 71
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1111/tmi.12248
ISSN:
13602276
e-ISSN:
13653156
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Tanzania