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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Abnormal methylation of KCNQ1OT1 and differential methylation of H19 imprinting control regions in human ICSI embryos
Zygote, Volume 21, No. 2, Year 2013
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Description
Summary To evaluate the integrity of genomic imprinting in embryos that failed to develop normally following intracytoplasmic sperm injection (ICSI), we analysed the methylation profile of H19 and KCNQ1OT1 imprinting control regions, H19DMR and KvDMR1 respectively, in high-grade blastocysts and in embryos that exhibited developmental anomalies. Significant hypomethylation of KvDMR1 was specifically observed in 5/5 atypical blastocysts graded BC, which probably reflected the vulnerability of the imprint in the inner cell mass during the methylation remodelling phase in the early embryo. In addition, KvDMR1 was hypermethylated in 2/5 CC graded atypical blastocysts and in 2/8 embryos that exhibited developmental delay. H19DMR appeared differentially methylated in all groups of embryos. DNA methyltransfersase 1 (DNMT1) expression was similar in most of the tested embryos and could not account for the abnormal methylation patterns of KvDMR1 observed. © Cambridge University Press 2012.
Authors & Co-Authors
Khoueiry, Rita
France, Bron
Institut Cellule Souche et Cerveau
Ibala-Romdhane, S.
Tunisia, Sousse
Hopital Farhat Hached Sousse
Al-Khtib, Mohamed
France, Bron
Institut Cellule Souche et Cerveau
Blachère, Thierry
France, Bron
Institut Cellule Souche et Cerveau
Lornage, Jacqueline
France, Bron
Institut Cellule Souche et Cerveau
France, Bron
Hôpital Femme
Guérin, Jean François
France, Bron
Institut Cellule Souche et Cerveau
France, Bron
Hôpital Femme
Lefèvre, Annick Elisabeth
France, Bron
Institut Cellule Souche et Cerveau
Statistics
Citations: 23
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1017/S0967199411000694
ISSN:
09671994
Research Areas
Genetics And Genomics