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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Cell screening assay for identifying inhibitors of eosinophil proliferation
Drug Development Research, Volume 72, No. 4, Year 2011
Notification
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Description
The purpose of this study was to develop a cell-based screening assay for identification of small molecules for the treatment of asthma. Eosinophils are leukocytes that contribute to the pathology of asthma. Lidocaine inhibits interleukin-5 (IL-5)-mediated survival and activation of human eosinophils, and it is able to replace inhaled glucocorticoids for the treatment of asthma; however, lidocaine has many side effects, including anesthesia. Therefore, a collection of commercial and novel, synthesized lidocaine analogues were investigated for inhibitory activity of the IL-5-stimulated proliferation of TF-1 cells, a CD34+, cytokine-dependent, erythroleukemic cell line model for eosinophil growth. Among 74 investigated compounds, 10 were more potent inhibitors of cell proliferation than lidocaine (average IC50 = 223 μM), with IC50 values ranging within 1-119 μM. This cell-based assay is an effective method for screening chemical compounds and has revealed promising lead compounds for the treatment of asthma. © 2011 Wiley-Liss, Inc.
Authors & Co-Authors
Kempe-Dustin, Jessica J.
United States, Salt Lake City
The University of Utah
Aboul-Fadl, Tarek
Saudi Arabia, Riyadh
King Saud University
Egypt, Asyut
Assiut University
Christensen, Clarissa
United States, Salt Lake City
The University of Utah
Palais, Robert
United States, Salt Lake City
The University of Utah
Parsawar, Krishna
United States, Salt Lake City
The University of Utah
Gleich, Gerald J.
United States, Salt Lake City
The University of Utah
Wagner, Lori A.
United States, Salt Lake City
The University of Utah
Statistics
Citations: 7
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1002/ddr.20438
ISSN:
02724391
e-ISSN:
10982299