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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Characterisation and serology of virus‐like particles associated with faba bean necrotic yellows
Annals of Applied Biology, Volume 123, No. 3, Year 1993
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Description
A disease showing chlorosis, leaf rolling and stunting in Vicia faba and other legumes was observed in West Asia and North Africa during 1987–1988. The putative causal agent could not be transmitted mechanically, but could be transmitted by aphids, most efficiently by Acyrthosiphon pisum, in the persistent manner. Further studies revealed isometric virus‐like particles (VLPs) closely associated with the disease, although their infectivity could not be demonstrated by membrane feeding. These particles, measuring c. 18 nm in diameter and containing a capsid protein of about 22 kDa and ssDNA of about 1 kb, are hereafter designated faba bean necrotic yellows virus (FBNYV). A high proportion of circular nucleic acid molecules of about 0.9 kb were visualised by electron microscopy. Hybridisation analysis of cloned viral DNA suggests that the circular genome is larger than 1 kb and consists of several components of similar size. An antiserum produced against FBNYV was used in ELISA, immunoelectron microscopy (IEM) and Western blot experiments for virus detection in aphids and field samples and for serological comparison with other viruses. Weak heterologous reactions between FBNYV and subterranean clover stunt virus (SCSV) were detected in IEM, but could not be confirmed in ELISA or Western blots. No serological relationship to banana bunchy top virus (BBTV) was detected. Using a direct tissue blot immunoassay (TBIA), FBNYV was detected in vascular tissue of infected faba bean leaves and stems. Copyright © 1993, Wiley Blackwell. All rights reserved
Authors & Co-Authors
KATUL, UNA
Germany, Quedlinburg
Julius Kühn-institut - Federal Research Centre for Cultivated Plants
Vetten, Heinrich Josef
Germany, Quedlinburg
Julius Kühn-institut - Federal Research Centre for Cultivated Plants
Maiß, Edgar
Germany, Quedlinburg
Julius Kühn-institut - Federal Research Centre for Cultivated Plants
Makkouk, Khaled M.
Lebanon, Beirut
International Center for Agricultural Research in the Dry Areas Syria
Lesemann, Dietrich Eckhardt
Germany, Quedlinburg
Julius Kühn-institut - Federal Research Centre for Cultivated Plants
Casper, R.
Germany, Quedlinburg
Julius Kühn-institut - Federal Research Centre for Cultivated Plants
Statistics
Citations: 79
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1111/j.1744-7348.1993.tb04933.x
ISSN:
00034746
e-ISSN:
17447348
Research Areas
Food Security
Genetics And Genomics
Study Locations
Multi-countries