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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
An evaluation of commercial fluorescent bead-based luminex cytokine assays
PLoS ONE, Volume 3, No. 7, Article e2535, Year 2008
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Description
The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 μl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex cytokine fluorescent bead-based immunoassays (Bio-Rad's Cytokine 17-piex kit; LINCO Inc's 29-plex kit; and RnD System's Fluorokine-Multi Analyte Profiling (MAP) base kit A and B). The LINEO Inc kit was found to be the most sensitive assay for measuring concentrations of multiple recombinant cytokines in samples that had been spiked with serial dilutions of the standard provided by the manufacturer, followed respectively by the RnD Fluorokine-(MAP) and Bio-Rad 17-plex kits. A positive correlation was found in the levels of IFN-γ measured in antigen stimulated whole blood culture supernatants by the UNCO inc 29-plex, RnD Fluorokine-(MAP) and RnD system IFN-γ Quantikine ELISA kits across a panel of controls and stimulated samples. Researchers should take the limitation of such multiplexed assays into account when planning experiments and the most appropriate use for these tests may currently be as screening tools for the selection of promising markers for analysis by more sensitive techniques. © 2008 Djoba Siawaya et al.
Authors & Co-Authors
Djoba-Siaway, Joël Fleury
South Africa, Stellenbosch
Stellenbosch University
Roberts, Teri R.
South Africa, Stellenbosch
Stellenbosch University
Babb, Chantal L.
South Africa, Stellenbosch
Stellenbosch University
Black, Gillian Frances
South Africa, Stellenbosch
Stellenbosch University
Golakai, Hawa Jande
South Africa, Stellenbosch
Stellenbosch University
Stanley, Kim
South Africa, Stellenbosch
Stellenbosch University
Bapela, Nchinya Bennedict
South Africa, Stellenbosch
Stellenbosch University
Hoal, Eileen G.
South Africa, Stellenbosch
Stellenbosch University
Parida, Shreemanta K.
Germany, Berlin
Max Planck Institute for Infection Biology
Van Helden, Paul D.
South Africa, Stellenbosch
Stellenbosch University
Walzl, Gerhard
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 166
Authors: 11
Affiliations: 2
Identifiers
Doi:
10.1371/journal.pone.0002535
e-ISSN:
19326203