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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Isolation of a mutant LLC‐PK
1
cell line defective in hormonal responsiveness: A pleiotropic lesion in receptor function
European Journal of Biochemistry, Volume 160, No. 2, Year 1986
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Description
A mutant LLC‐PK1 cell line, M18, was isolated after a single treatment of the parent culture with N‐methyl‐N′‐nitro‐N‐nitroso‐guanidine. In contrast to LLC‐PK1 cells, the mutant did not exhibit production of urokinasetype plasminogen activator (uPA) in response to the hormones calcitonin and vasopressin, but produced the expected levels of uPA upon stimulation by the receptor‐independent adenylate cyclase activators forskolin and cholera toxin, as well as by the phosphodiesterase inhibitor isobutylmethylxanthine and the 8‐bromo analogue of adenosine cyclic monophosphate, Br8cAMP. The patterns of activation of cAMP‐dependent protein kinase were identical to those of uPA induction: calcitonin and vasopressin were without effect, but the response to all other agents was normal. In similar fashion, mutant cell homogenates displayed normal activation of adenylate cyclase upon treatment with sodium fluoride, forskolin, or the non‐hydrolyzable GTP analogue guanosine 5′‐[β,γ‐imino]triphosphate, but were unresponsive to calcitonin or vasopressin. The ability of M18 cells to bind radioactively labelled calcitonin and vasopressin was measured. The mutant possessed less than 4% of the normal levels of the receptor binding activity for both hormones. Somatic cell hybrids formed between M18 and LLC‐PK1 cells were found to retain normal hormone binding activity and responsiveness to hormones, indicating that the defect in M18 cells was recessive. M18 was concluded most probably to contain a single mutation impairing the function of two distinct polypeptide hormone receptors. Copyright © 1986, Wiley Blackwell. All rights reserved
Authors & Co-Authors
JANS, David A.
Switzerland, Basel
Friedrich Miescher Institute for Biomedical Research
RESINK, Therese J.
Switzerland, Basel
Universitätsspital Basel
WILSON, Lynette E.
South Africa, Cape Town
Medical School
Reich, Edward
Switzerland, Basel
Roche Pharmaceuticals Division
HEMMINGS, Brian A.
Switzerland, Basel
Friedrich Miescher Institute for Biomedical Research
Statistics
Citations: 26
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1111/j.1432-1033.1986.tb09986.x
ISSN:
00142956
e-ISSN:
14321033
Research Areas
Cancer
Infectious Diseases