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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Insulin-producing cells from adult human bone marrow mesenchymal stem cells control streptozotocin-induced diabetes in nude mice
Cell Transplantation, Volume 22, No. 1, Year 2013
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Description
Harvesting, expansion, and directed differentiation of human bone marrow-derived mesenchymal stem cells (BM-MSCs) could provide an autologous source of surrogate β-cells that would alleviate the limitations of availability and/or allogenic rejection following pancreatic or islet transplantation. Bone marrow cells were obtained from three adult type 2 diabetic volunteers and three nondiabetic donors. After 3 days in culture, adherent MSCs were expanded for two passages. At passage 3, differentiation was carried out in a three-staged procedure. Cells were cultured in a glucose-rich medium containing several activation and growth factors. Cells were evaluated in vitro by flow cytometry, immunolabeling, RT-PCR, and human insulin and c-peptide release in responses to increasing glucose concentrations. One thousand cell clusters were inserted under the renal capsule of diabetic nude mice followed by monitoring of their diabetic status. At the end of differentiation, ~5-10% of cells were immunofluorescent for insulin, c-peptide or glucagon; insulin, and c-peptide were coexpressed. Nanogold immunolabeling for electron microscopy demonstrated the presence of c-peptide in the rough endoplasmic reticulum. Insulin-producing cells (IPCs) expressed transcription factors and genes of pancreatic hormones similar to those expressed by pancreatic islets. There was a stepwise increase in human insulin and c-peptide release by IPCs in response to increasing glucose concentrations. Transplantation of IPCs into nude diabetic mice resulted in control of their diabetic status for 3 months. The sera of IPC-transplanted mice contained human insulin and c-peptide but negligible levels of mouse insulin. When the IPC-bearing kidneys were removed, rapid return of diabetic state was noted. BM-MSCs from diabetic and nondiabetic human subjects could be differentiated without genetic manipulation to form IPCs that, when transplanted, could maintain euglycemia in diabetic mice for 3 months. Optimization of the culture conditions are required to improve the yield of IPCs and their functional performance. © 2013 Cognizant Comm. Corp.
Authors & Co-Authors
Gabr, Mahmoud M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Zakaria, Mahmoud M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Refaie, Ayman Fathi
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Ismail, A. M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Abdel-Rahim, Mona
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Ashamallah, Sylvia A.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Khater, Sherry M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
El-Halawani, Sawsan M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Ibrahim, Rana Y.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Gan, Shu Uin
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Kloc, Malgorzata
United States, Houston
Houston Methodist Hospital
Calne, Roy Yorke
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
United Kingdom, Cambridge
University of Cambridge
Ghoneim, Mohamed A.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Statistics
Citations: 99
Authors: 13
Affiliations: 4
Identifiers
Doi:
10.3727/096368912X647162
ISSN:
09636897
Research Areas
Cancer
Genetics And Genomics
Noncommunicable Diseases