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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Comparison of ELISA and immunoassays for measurement of IgG and IgM antibody to West Nile virus in human sera against virus neutralisation
Journal of Virological Methods, Volume 139, No. 1, Year 2007
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Description
Two commercial assays for the detection of IgG antibody to West Nile virus (WNV), an indirect enzyme-linked immunosorbent assay (I-ELISA) and indirect fluorescent antibody test (IFAT), were evaluated against the virus neutralisation test. Excellent agreement with the virus neutralisation was obtained with both tests, i.e., 99.5% by I-ELISA and 100% by IFAT. The well-known serological cross-reactivity within the family of the Flaviviridae was analysed using sera with known antibodies against dengue virus, tick borne encephalitis virus and yellow fever virus. IgM and/or IgG positive sera were examined for reactivity by WNV-ELISA and WNV-IFAT. While cross-reactivity between 0 and 18.2% was recorded with IgM positive sera, there was extensive cross-reactivity of 15.7-100% with IgG positive sera. © 2006 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Niedrig, Matthias
Germany, Berlin
Robert Koch Institute
Sonnenberg, K.
Germany, Lubeck
Euroimmun ag
Steinhagen, Katja
Germany, Lubeck
Euroimmun ag
Pawȩska, Janusz Tadeusz
South Africa, Johannesburg
National Institute for Communicable Diseases
Statistics
Citations: 61
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1016/j.jviromet.2006.09.009
ISSN:
01660934
Research Areas
Infectious Diseases