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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Different mechanisms for phytoalexin induction by pathogen and wound signals in Medicago truncatula
Proceedings of the National Academy of Sciences of the United States of America, Volume 104, No. 46, Year 2007
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Description
Cell suspensions of the model legume Medicago truncatula accumulated the isoflavonoid phytoalexin medicarpin in response to yeast elicitor or methyl jasmonate (MJ), accompanied by decreased levels of isoflavone glycosides in MJ-treated cells. DNA microarray analysis revealed rapid, massive induction of early (iso)flavonoid pathway gene transcripts in response to yeast elicitor, but not MJ, and differential induction by the two elicitors of sets of genes encoding transcription factors, ABC transporters, and β-glucosidases. In contrast, both elicitors induced genes encoding enzymes for conversion of the isoflavone formononetin to medicarpin. Four MJ-induced β-glucosidases were expressed as recombinant enzymes in yeast, and three were active with isoflavone glucosides. The most highly induced β-glucosidase was nuclear localized and preferred flavones to isoflavones. The results indicate that the genetic and biochemical mechanisms underlying accumulation of medicarpin differ depending on the nature of the stimulus and suggest a role for MJ as a signal for rapid hydrolysis of preformed, conjugated intermediates for antimicrobial biosynthesis during wound responses. © 2007 by The National Academy of Sciences of the USA.
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Authors & Co-Authors
Naoumkina, Marina A.
United States, Ardmore
Samuel Roberts Noble Foundation
Farag, Mohamed Ali
United States, Ardmore
Samuel Roberts Noble Foundation
Egypt, Cairo
Faculty of Pharmacy
Sumner, Lloyd W.
United States, Ardmore
Samuel Roberts Noble Foundation
Tang, Yuhong
United States, Ardmore
Samuel Roberts Noble Foundation
Liu, Chang Jun
United States, Upton
Brookhaven National Laboratory
Dixon, Richard Arthur
United States, Ardmore
Samuel Roberts Noble Foundation
Statistics
Citations: 177
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1073/pnas.0708697104
ISSN:
00278424
Research Areas
Cancer
Genetics And Genomics