Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Genome-wide functional analysis of plasmodium protein phosphatases reveals key regulators of parasite development and differentiation
Cell Host and Microbe, Volume 16, No. 1, Year 2014
Notification
URL copied to clipboard!
Description
Reversible protein phosphorylation regulated by kinases and phosphatases controls many cellular processes. Although essential functions for the malaria parasite kinome have been reported, the roles of most protein phosphatases (PPs) during Plasmodium development are unknown. We report a functional analysis of the Plasmodium berghei protein phosphatome, which exhibits high conservation with the P. falciparum phosphatome and comprises 30 predicted PPs with differential and distinct expression patterns during various stages of the life cycle. Gene disruption analysis of P. berghei PPs reveals that half of the genes are likely essential for asexual blood stage development, whereas six are required for sexual development/sporogony in mosquitoes. Phenotypic screening coupled with transcriptome sequencing unveiled morphological changes and altered gene expression in deletion mutants of two N-myristoylated PPs. These findings provide systematic functional analyses of PPs in Plasmodium, identify how phosphatases regulate parasite development and differentiation, and can inform the identification of drug targets for malaria. © 2014 The Authors.
Authors & Co-Authors
Guttery, David S.
United Kingdom, Nottingham
University of Nottingham
United Kingdom, Leicester
University of Leicester
Poulin, Benoit
United Kingdom, Nottingham
University of Nottingham
Ramaprasad, Abhinay
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Wall, Richard J.
United Kingdom, Nottingham
University of Nottingham
Ferguson, David J. P.
United Kingdom, Oxford
University of Oxford Medical Sciences Division
Brady, Declan
United Kingdom, Nottingham
University of Nottingham
Patzewitz, Eva Maria
United Kingdom, Nottingham
University of Nottingham
Whipple, Sarah
United Kingdom, Nottingham
University of Nottingham
Straschil, Ursula
United Kingdom, London
Imperial College London
Wright, Megan H.
United Kingdom, London
Imperial College London
Mohamed, Alyaa M.A.H.
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Radhakrishnan, Anand K.
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Arold, Stefan T.
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Tate, Edward W.
United Kingdom, London
Imperial College London
Holder, Anthony A.
United Kingdom, London
Mrc National Institute for Medical Research
Wickstead, Bill
United Kingdom, Nottingham
University of Nottingham
Pain, Arnab P.
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Tewari, Rita
United Kingdom, Nottingham
University of Nottingham
Statistics
Citations: 107
Authors: 18
Affiliations: 6
Identifiers
Doi:
10.1016/j.chom.2014.05.020
ISSN:
19313128
e-ISSN:
19346069
Research Areas
Genetics And Genomics
Infectious Diseases
Sexual And Reproductive Health