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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Cholesterol diet-induced hyperlipidemia impairs the cardioprotective effect of postconditioning: Role of peroxynitrite
American Journal of Physiology - Heart and Circulatory Physiology, Volume 297, No. 5, Year 2009
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Description
The aim of the present study was to investigate if hyperlipidemia interferes with the infarct size-limiting effect of postconditioning and to study the involvement of peroxynitrite in this phenomenon. Rats were fed a 2% cholesterol-enriched or normal diet for 12 wk. Infarct size by triphenyltetrazolium chloride staining was measured in hearts isolated from both groups and subjected to 30 min coronary occlusion followed by 120 min reperfusion with or without the postconditioning protocol induced by six cycles of 10 s coronary occlusion and 10 s reperfusion at the onset of the reperfusion. Postconditioning significantly decreased infarct size in the normolipidemic but not in the hyperlipidemic group. Postconditioning increased cardiac 3-nitrotyrosine concentration (a marker for peroxynitrite formation) in the normal but not in the cholesterol-fed group when measured at the 5th min of reperfusion. Next, we tested if the postconditioning-induced acute increase in peroxynitrite is involved in the cardioprotection in normolipidemic animals in separate experiments. Postconditioning failed to decrease infarct size in the presence of the peroxynitrite decomposition catalyst 5,10,15,20-tetrakis-[4- sulfonatophenyl]-porphyrinato-iron [III] (20 mg/l) in normolipidemic animals. We conclude that an early increase in peroxynitrite after postconditioning plays a role in cardioprotection. Furthermore, hyperlipidemia blocks the cardioprotective effect of postconditioning at least in part via deterioration of the postconditioning-induced early increase in peroxynitrite formation. Copyright © 2009 the American Physiological Society.
Authors & Co-Authors
Kupai, Krisztina
Hungary, Szeged
Cardiovascular Research Group, Szeged
Hungary, Budapest
Pharmahungary Group
Csonka, Csaba
Hungary, Szeged
Cardiovascular Research Group, Szeged
Hungary, Budapest
Pharmahungary Group
Fekete, Veronika
Hungary, Szeged
Cardiovascular Research Group, Szeged
Hungary, Budapest
Pharmahungary Group
Odendaal, Louise
South Africa, Bellville
Cape Peninsula University of Technology
van Rooyen, Jacques
South Africa, Bellville
Cape Peninsula University of Technology
Marais, De Wet
South Africa, Tygerberg
South African Medical Research Council
Csont, Tamás
Hungary, Szeged
Cardiovascular Research Group, Szeged
Hungary, Budapest
Pharmahungary Group
Ferdinandy, Peter
Hungary, Szeged
Cardiovascular Research Group, Szeged
Hungary, Budapest
Pharmahungary Group
Statistics
Citations: 95
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1152/ajpheart.00484.2009
ISSN:
03636135
e-ISSN:
15221539
Research Areas
Noncommunicable Diseases