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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Evaluation of Flinders Technology Associates cards for storage and molecular detection of avian metapneumoviruses
Avian Pathology, Volume 43, No. 2, Year 2014
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Description
The feasibility of using Flinders Technology Associates (FTA) cards for the molecular detection of avian metapneumovirus (aMPV) by reverse transcriptase-polymerase chain reaction (RT-PCR) was investigated. Findings showed that no virus isolation was possible from aMPV-inoculated FTA cards, confirming viral inactivation upon contact with the cards. The detection limits of aMPV from the FTA card and tracheal organ culture medium were 101.5 median ciliostatic doses/ml and 100.75 median ciliostatic doses/ml respectively. It was possible to perform molecular characterization of both subtypes A and B aMPV using inoculated FTA cards stored for up to 60 days at 4 to 6°C. Tissues of the turbinate, trachea and lung of aMPV-infected chicks sampled either by direct impression smears or by inoculation of the tissue homogenate supernatants onto the FTA cards were positive by RT-PCR. However, the latter yielded more detections. FTA cards are suitable for collecting and transporting aMPV-positive samples, providing a reliable and hazard-free source of RNA for molecular characterization. © 2014 © 2014 Houghton Trust Ltd.
Authors & Co-Authors
Awad, Faez
United Kingdom, Liverpool
University of Liverpool
Libya, Al Bayda
Omar Al-mukhtar University
Baylis, Matthew
United Kingdom, Liverpool
University of Liverpool
Jones, Richard Charles
United Kingdom, Liverpool
University of Liverpool
Ganapathy, Kannan K.
United Kingdom, Liverpool
University of Liverpool
Statistics
Citations: 24
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1080/03079457.2014.885114
ISSN:
03079457
e-ISSN:
14653338
Research Areas
Environmental