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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Cattle immunized against the pathogenic l-α-glycerol-3-phosphate oxidase of Mycoplasma mycoides subs. mycoides fail to generate neutralizing antibodies and succumb to disease on challenge
Vaccine, Volume 31, No. 44, Year 2013
Notification
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Description
The membrane-associated enzyme l-α-glycerol-3-phosphate oxidase (GlpO) of Mycoplasma mycoides subs. mycoides (Mmm), the causal agent of contagious bovine pleuropneumonia (CBPP) has been identified as a virulence factor responsible for the release of toxic by-products such as H2O2 that mediate host cell injury. Since CBPP pathogenesis is based on host inflammatory reactions, we have determined the capacity of recombinant GlpO to generate in vivo protective responses against challenge in immunized cattle. We also investigated whether sera raised against recombinant GlpO in cattle and mice inhibit production of H2O2 by Mmm. Immunization of cattle with recombinant GlpO did not protect against challenge with a virulent strain of Mmm. Further, although both murine and bovine antisera raised against recombinant GlpO detected recombinant and native forms of GlpO in immunoblot assays with similar titres, only murine antibodies could neutralize GlpO enzymatic function. The data raise the possibility that Mmm has adapted to evade potential detrimental antibody responses in its definitive host. © 2013 The Authors.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3989769/bin/mmc1.kml
Authors & Co-Authors
Mulongo, Musa M.
Kenya, Nairobi
International Livestock Research Institute Nairobi
United Kingdom, London
Royal Veterinary College University of London
Frey, Joachim
Switzerland, Bern
University of Bern
Smith, Kenneth C.
United Kingdom, London
Royal Veterinary College University of London
Schnier, Christian
United Kingdom, Midlothian
Moredun Research Institute
Wesonga, Hezron Okwako
Kenya, Nairobi
National Veterinary Research Center
Naessens, Jan
Kenya, Nairobi
International Livestock Research Institute Nairobi
McKeever, Declan J.
United Kingdom, London
Royal Veterinary College University of London
Statistics
Citations: 17
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1016/j.vaccine.2013.08.100
ISSN:
0264410X
e-ISSN:
18732518
Research Areas
Environmental
Maternal And Child Health
Violence And Injury