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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Molecular epidemiology of wild poliovirus type 1 circulation in West and Central Africa, from 1997 to 1999, using genotyping with a restriction fragment length polymorphism assay
Archives of Virology, Volume 153, No. 3, Year 2008
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Description
Virological surveillance is an important element in the Polio Eradication Initiative to provide information rapidly about circulating wild polioviruses. Molecular tools have been developed to identify the serotype of the poliovirus strains and whether they are of vaccine or wild origin (intratypic differentiation) and to perform the molecular epidemiology of wild strains. The main objective of this study was to show that restriction fragment length polymorphism (RFLP) is a tool that can be used for molecular epidemiology of wild polioviruses. This is retrospective study of poliovirus type 1 strains received at the Institut Pasteur of Bangui (IPB), a WHO Regional Reference Laboratory for Africa, since 1994. We describe our experience with isolates from Western and Central Africa and show a positive correlation between the genotypes as determined by sequencing the gene for the VP1 capsid protein and the RFLP patterns. Although genomic sequencing is the gold standard method for detailed molecular epidemiology analysis of poliovirus isolates, these results show that RFLP is a potentially valuable tool for molecular epidemiological analysis of poliovirus type 1 strains: it could be used by many laboratories as a rapid method for ITD and genotype screening where sequencing capacity is not readily available. © 2007 Springer-Verlag.
Authors & Co-Authors
Gouandjika-Vasilache, Ionela
Central African Republic, Bangui
Institut Pasteur de Bangui
Burns, Cara Carthel
United States, Atlanta
Centers for Disease Control and Prevention
Gumede, Nicksy
South Africa, Johannesburg
National Institute for Communicable Diseases
Guillot, Sophie
France, Paris
Institut Pasteur, Paris
Dosseh, Annick R.G.A.
Senegal, Dakar
Institut Pasteur de Dakar
Akoua-Koffl, Chantal Gnankon
Cote D'ivoire, Abidjan
Institut Pasteur de Cote-d'ivoire
Pallansch, Mark A.
United States, Atlanta
Centers for Disease Control and Prevention
Kew, Olen M.
United States, Atlanta
Centers for Disease Control and Prevention
Delpeyroux, Francis
France, Paris
Institut Pasteur, Paris
Statistics
Citations: 10
Authors: 9
Affiliations: 7
Identifiers
Doi:
10.1007/s00705-007-0001-x
ISSN:
03048608
Research Areas
Genetics And Genomics
Study Design
Cohort Study