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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Evaluation of the AID TB resistance line probe assay for rapid detection of genetic alterations associated with drug resistance in Mycobacterium tuberculosis strains
Journal of Clinical Microbiology, Volume 52, No. 3, Year 2014
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Description
The rapid accurate detection of drug resistance mutations in Mycobacterium tuberculosis is essential for optimizing the treatment of tuberculosis and limiting the emergence and spread of drug-resistant strains. The TB Resistance line probe assay from Autoimmun Diagnostika GmbH (AID) (Strassburg, Germany) was designed to detect the most prevalent mutations that confer resistance to isoniazid, rifampin, streptomycin, amikacin, capreomycin, fluoroquinolones, and ethambutol. This assay detected resistance mutations in clinical M. tuberculosis isolates from areas with low and high levels of endemicity (Switzerland, n=104; South Africa, n=52) and in selected Mycobacterium bovis BCG 1721 mutant strains (n=5) with 100% accuracy. Subsequently, the line probe assay was shown to be capable of rapid genetic assessment of drug resistance in MGIT broth cultures, the results of which were in 100% agreement with those of DNA sequencing and phenotypic drug susceptibility testing. Finally, the line probe assay was assessed for direct screening of smear-positive clinical specimens. Screening of 98 clinical specimens demonstrated that the test gave interpretable results for>95% of them. Antibiotic resistance mutations detected in the clinical samples were confirmed by DNA sequencing. We conclude that the AID TB Resistance line probe assay is an accurate tool for the rapid detection of resistance mutations in cultured isolates and in smear-positive clinical specimens. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Ritter, Claudia
Switzerland, Zurich
Universität Zürich
Switzerland, Zurich
Nationales Zentrum Für Mykobakterien
Lucke, K.
Switzerland, Zurich
Universität Zürich
Switzerland, Zurich
Nationales Zentrum Für Mykobakterien
Sirgel, Frederick Adriaan
South Africa, Stellenbosch
Stellenbosch University
Warren, Robin Mark
South Africa, Stellenbosch
Stellenbosch University
Van Helden, Paul D.
South Africa, Stellenbosch
Stellenbosch University
Böttger, Erik Christian
Switzerland, Zurich
Universität Zürich
Switzerland, Zurich
Nationales Zentrum Für Mykobakterien
Bloemberg, G. V.
Switzerland, Zurich
Universität Zürich
Statistics
Citations: 43
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1128/JCM.02597-13
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
South Africa