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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Downregulation of pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase activity in sugarcane culms enhances sucrose accumulation due to elevated hexose-phosphate levels
Planta, Volume 231, No. 3, Year 2010
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Description
Analyses of transgenic sugarcane clones with 45-95% reduced cytosolic pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase (PFP, EC 2. 7. 1. 90) activity displayed no visual phenotypical change, but significant changes were evident in in vivo metabolite levels and fluxes during internode development. In three independent transgenic lines, sucrose concentrations increased between three- and sixfold in immature internodes, compared to the levels in the wildtype control. There was an eightfold increase in the hexose-phosphate:triose-phosphate ratio in immature internodes, a significant restriction in the triose phosphate to hexose phosphate cycle and significant increase in sucrose cycling as monitored by 13C nuclear magnetic resonance. This suggests that an increase in the hexose-phosphate concentrations resulting from a restriction in the conversion of hexose phosphates to triose phosphates drive sucrose synthesis in the young internodes. These effects became less pronounced as the tissue matured. Decreased expression of PFP also resulted in an increase of the ATP/ADP and UTP/UDP ratios, and an increase of the total uridine nucleotide and, at a later stage, the total adenine nucleotide pool, revealing strong interactions between PPi metabolism and general energy metabolism. Finally, decreased PFP leads to a reduction of PPi levels in older internodes indicating that in these developmental stages PFP acts in the gluconeogenic direction. The lowered PPi levels might also contribute to the absence of increases in sucrose contents in the more mature tissues of transgenic sugarcane with reduced PFP activity. © 2009 The Author(s).
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2806535/bin/425_2009_1069_MOESM1_ESM.xls
Authors & Co-Authors
van der Merwe, Margaretha J.
South Africa, Stellenbosch
Stellenbosch University
Groenewald, Jan Hendrik
South Africa, Stellenbosch
Stellenbosch University
South Africa
Plantbio
Stitt, Mark
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Kossmann, J. M.
South Africa, Stellenbosch
Stellenbosch University
Botha, F. C.
South Africa, Stellenbosch
Stellenbosch University
Australia, Brisbane
Srdc
Statistics
Citations: 59
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1007/s00425-009-1069-1
ISSN:
00320935
e-ISSN:
14322048
Research Areas
Cancer