Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Global histone modifications in breast cancer correlate with tumor phenotypes, prognostic factors, and patient outcome
Cancer Research, Volume 69, No. 9, Year 2009
Notification
URL copied to clipboard!
Description
Post-translational histone modifications are known to be altered in cancer cells, and loss of selected histone acetylation and methylation marks has recently been shown to predict patient outcome in human carcinoma. Immunohistochemistry was used to detect a series of histone lysine acetylation (H3K9ac, H3K18ac, H4K12ac, and H4K16ac), lysine methylation (H3K4me2 and H4K20me3), and arginine methylation (H4R3me2) marks in a well-characterized series of human breast carcinomas (n = 880). Tissue staining intensities were assessed using blinded semiquantitative scoring. Validation studies were done using immunofluorescence staining and Western blotting. Our analyses revealed low or absent H4K16ac in the majority of breast cancer cases (78.9%), suggesting that this alteration may represent an early sign of breast cancer. There was a highly significant correlation between histone modifications status, tumor biomarker phenotype, and clinical outcome, where high relative levels of global histone acetylation and methylation were associated with a favorable prognosis and detected almost exclusively in luminal-like breast tumors (93%). Moderate to low levels of lysine acetylation (H3K9ac, H3K18ac, and H4K12ac), lysine (H3K4me2 and H4K20me3), and arginine methylation (H4R3me2) were observed in carcinomas of poorer prognostic subtypes, including basal carcinomas and HER-2-positive tumors. Clustering analysis identified three groups of histone displaying distinct pattern in breast cancer, which have distinct relationships to known prognostic factors and clinical outcome. This study identifies the presence of variations in global levels of histone marks in different grades, morphologic types, and phenotype classes of invasive breast cancer and shows that these differences have clinical significance. © 2009 American Association for Cancer Research.
Authors & Co-Authors
Elsheikh, Somaia Elbauomy
United Kingdom, Nottingham
University of Nottingham
Egypt, Shibin el Kom
Menoufia University Faculty of Medicine
Green, Andew R.
United Kingdom, Nottingham
University of Nottingham
Rakha, Emad A.
United Kingdom, Nottingham
University of Nottingham
Powe, Desmond George
United Kingdom, Nottingham
University of Nottingham
Ahmed, Rabab A.
United Kingdom, Nottingham
University of Nottingham
Egypt, Asyut
Faculty of Veterinary Medicine
Collins, Hilary M.
United Kingdom, Nottingham
School of Pharmacy
Soria, Daniele
United Kingdom, Nottingham
School of Computer Science
Garibaldi, Jonathan M.
United Kingdom, Nottingham
School of Computer Science
Paish, Claire E.
United Kingdom, Nottingham
University of Nottingham
Ammar, A. A.
Egypt, Shibin el Kom
Menoufia University Faculty of Medicine
Grainge, Matthew J.
United Kingdom, Nottingham
University of Nottingham Medical School
Ball, Graham Roy
United Kingdom, Nottingham
Nottingham Trent University
Abdelghany, Magdy K.
United Kingdom, Nottingham
School of Pharmacy
Egypt, Ismailia
Faculty of Medicine
Martinez-Pomares Luisa, Luisa
United Kingdom, Nottingham
University of Nottingham
Heery, David M.
United Kingdom, Nottingham
School of Pharmacy
Ellis, Ian O.
United Kingdom, Nottingham
University of Nottingham
United Kingdom, Nottingham
Nottingham University Hospitals Nhs Trust
Statistics
Citations: 430
Authors: 16
Affiliations: 9
Identifiers
Doi:
10.1158/0008-5472.CAN-08-3907
ISSN:
00085472
e-ISSN:
15387445
Research Areas
Cancer
Health System And Policy