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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Engineering of the α-amylase from geobacillus stearothermophilus US100 for detergent incorporation
Biotechnology and Bioengineering, Volume 102, No. 2, Year 2009
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Description
AmyUS100ΔIG is a variant of the most thermoactive and thermostable maltohexaose forming α-amylase produced by Geobacillus stearothermophilus sp.US100. This enzyme which was designed to improve the thermostability of the wild-type enzyme has acquired a very high resistance to chelator agents. According to modeling structural studies and with the aim of enhancing its resistance towards chemical oxidation, a mutant (AmyUS100ΔIG/M197A) was created by substituting methionine 197 to alanine. The catalytic proprieties of the resulting mutant show alterations in the specific activity and the profile of starch hydrolysis. Interestingly, AmyUS100ΔIG/M197A displayed the highest resistance to oxidation compared to the AmyUS100ΔIG and to Termamyl300®, the well-known commercial amylase used in detergent. Further, performance of the engineered α-amylase was estimated in the presence of commonly used detergent compounds and a wide range of commercial detergent (liquid and solid). These studies indicated a high compatibility and performance of AmyUS100ΔIG/M197A, suggesting its potential application in detergent industry. © 2008 Wiley Periodicals, Inc.
Authors & Co-Authors
Khemakhem, Bassem
Tunisia, Sfax
Centre de Biotechnologie de Sfax
France, Lyon
Institut de Biologie et Chimie Des Protéines
Ben Ali, Mamdouh
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Aghajari, Nushin
France, Lyon
Institut de Biologie et Chimie Des Protéines
Juy, Michel
France, Lyon
Institut de Biologie et Chimie Des Protéines
Haser, Richard
France, Lyon
Institut de Biologie et Chimie Des Protéines
Bejar, S.
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Statistics
Citations: 51
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1002/bit.22083
ISSN:
00063592