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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations
Nature Communications, Volume 5, Article 4767, Year 2014
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Description
The HEK293 human cell lineage is widely used in cell biology and biotechnology. Here we use whole-genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response to the manipulations used to generate common 293 cell derivatives, such as transformation and stable clone generation (293T); suspension growth adaptation (293S); and cytotoxic lectin selection (293SG). Remarkably, we observe that copy number alteration detection could identify the genomic region that enabled cell survival under selective conditions (i.c. ricin selection). Furthermore, we present methods to detect human/vector genome breakpoints and a user-friendly visualization tool for the 293 genome data. We also establish that the genome structure composition is in steady state for most of these cell lines when standard cell culturing conditions are used. This resource enables novel and more informed studies with 293 cells, and we will distribute the sequenced cell lines to this effect. © 2014 Macmillan Publishers Limited. All rights reserved.
Available Materials
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https://efashare.b-cdn.net/share/pmc/articles/PMC4166678/bin/ncomms5767-s2.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC4166678/bin/ncomms5767-s3.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC4166678/bin/ncomms5767-s4.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4166678/bin/ncomms5767-s5.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC4166678/bin/ncomms5767-s6.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC4166678/bin/ncomms5767-s7.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4166678/bin/ncomms5767-s8.pdf
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Authors & Co-Authors
Lin, Yao Cheng
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Boone, Morgane
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Meuris, Leander
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Lemmens, Irma
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Ghent, Faculteit Geneeskunde en Gezondheidswetenschappen
Van Roy, Nadine
Belgium, Ghent
Universitair Ziekenhuis Gent
Soete, Arne
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Reumers, Joke
Belgium, Leuven
Departement Menselijke Erfelijkheid
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Moisse, Matthieu
Belgium, Leuven
Departement Menselijke Erfelijkheid
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Plaisance, Stéphane
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Drmanac, Radoje
United States, Mountain View
Complete Genomics Inc.
China, Shenzhen
Bgi-shenzhen
Chen, Jason
United States, Mountain View
Complete Genomics Inc.
Speleman, Frank
Belgium, Ghent
Universitair Ziekenhuis Gent
Lambrechts, Diether
Belgium, Leuven
Departement Menselijke Erfelijkheid
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Van de Peer, Y.
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
South Africa, Pretoria
University of Pretoria
Tavernier, Jan
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Ghent, Faculteit Geneeskunde en Gezondheidswetenschappen
Callewaert, Nico
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Ghent
Universiteit Gent
Statistics
Citations: 439
Authors: 16
Affiliations: 8
Identifiers
Doi:
10.1038/ncomms5767
e-ISSN:
20411723