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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Laminar chemokine mRNA concentrations in horses with carbohydrate overload-induced laminitis
Veterinary Immunology and Immunopathology, Volume 144, No. 1-2, Year 2011
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Description
Chemokines play a vital role in leukocyte activation and emigration that reportedly plays a central role in laminar injury in equine laminitis. The purpose of this study was to evaluate the pattern of laminar chemokine expression in horses in the classical carbohydrate overload (CHO)-model of laminitis. Laminar samples were obtained 24. h following water administration in the control group (CON, n= 8), and at the onset of fever (≥102 °F, 12-22. h post CHO, DEV group, n= 8) and at the onset of lameness (20-48. h post CHO, LAM group, n= 8) in induced horses. Real time quantitative PCR was performed on all samples in order to determine laminar mRNA concentrations of both CXC chemokines (CXCL1, CXCL6, CXCL8) and CC chemokines (CCL2 [MCP-1], CCL3 [MIP-1α], and CCL8 [MCP-2]). Data were subjected to ANOVA followed by Student-Newman-Keuls (P< 0.05). Laminar mRNA concentrations for all CXC chemokines were increased (P< 0.05) at both the DEV and LAM horses when compared to the control horses, whereas mRNA concentrations of CCL2 and CCL8 were only increased in the LAM horses when compared to controls and the DEV horses. When taken in context with our previous studies, CXCL1, CXCL6 and CXCL8 increases precede peak laminar leukocyte accumulation. Additionally, CCL2 and CCL8 expression corroborate previous reports of monocyte/macrophage accumulation in affected laminae. Compared with previous studies, our findings demonstrate that increased laminar CXC chemokine expression consistently precedes peak leukocyte accumulation and onset of lameness in CHO laminitis models. Chemokine antagonists may be considered as possible therapeutic targets to decrease the influx of leukocytes that occurs during the development of equine laminitis. © 2011.
Authors & Co-Authors
Faleiros, Rafael R.
United States, Columbus
The Ohio State University
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Johnson, Philip James
United States, Columbia
Mu College of Veterinary Medicine
Black, Samuel J.
United States, Amherst
University of Massachusetts Amherst
Statistics
Citations: 39
Authors: 3
Affiliations: 4
Identifiers
Doi:
10.1016/j.vetimm.2011.07.005
ISSN:
18732534
Research Areas
Environmental
Violence And Injury
Study Design
Randomised Control Trial
Study Approach
Quantitative