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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Acquisition of callogenic capacity in date palm leaf tissues in response to 2,4-D treatment
Plant Cell, Tissue and Organ Culture, Volume 99, No. 1, Year 2009
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Description
While callogenesis in date palm can be initiated by culturing immature leaf segments on medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), it is difficult to obtain callus from certain genotypes. To better understand the mechanisms of callogenesis in date palm, we studied the developmental events underlying this process at the cellular level. The callogenic capacity of leaf segments depends on the state of cell differentiation. The most callogenic segments were within the leaf elongation zone, required polar auxin transport to initiate callogenesis and contained the highest quantities of free endogenous indole-3-acetic acid. At the cellular level, callus induction involves two spatially and temporally separated events. The first event involves cells from the fascicular parenchyma in an equatorial plane perpendicular to the vascular axis that within 2 days of culture in the presence of 2,4-D, acquire structural and ultrastructural features typically observed in meristematic cells. The second event occurs 3 days later and is characterised by the modification of adjacent perivascular sheath cells. The latter cells become callogenic in that they reinitiated their cell cycles and undergo cell division leading to callus formation. These data provide evidence that callus initiation in leaf vascular tissue of date palm involves a sequential response of two distinct cell types to auxin and requires polar auxin transport. © Springer Science+Business Media B.V. 2009.
Authors & Co-Authors
Guéye, Badara
France, Montpellier
Ird Centre de Montpellier
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Nigeria, Ibadan
International Institute of Tropical Agriculture
Morcillo, F.
France, Montpellier
Ird Centre de Montpellier
Collin, Myriam
France, Montpellier
Ird Centre de Montpellier
Gargani, D.
France, Paris
Cirad
Overvoorde, Paul
United States, Minneapolis
Macalester College
Aberlenc-Bertossi, Frédérique
France, Montpellier
Ird Centre de Montpellier
Tranbarger, Timothy John
France, Montpellier
Ird Centre de Montpellier
Sané, Djibril
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Tregear, James W.
France, Montpellier
Ird Centre de Montpellier
Borg̀el, Alain
France, Montpellier
Ird Centre de Montpellier
Verdeil, Jean Luc
France, Paris
Cirad
Statistics
Citations: 51
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1007/s11240-009-9573-3
ISSN:
01676857