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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Proposal of a consensus set of hypervariable mycobacterial interspersed repetitive-unit-variable-number tandem-repeat loci for subtyping of mycobacterium tuberculosis Beijing isolates
Journal of Clinical Microbiology, Volume 52, No. 1, Year 2014
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Description
Mycobacterium tuberculosis Beijing strains represent targets of special importance for molecular surveillance of tuberculosis (TB), especially because they are associated with spread of multidrug resistance in some world regions. Standard 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing lacks resolution power for accurately discriminating closely related clones that often compose Beijing strain populations. Therefore, we evaluated a set of 7 additional, hypervariable MIRU-VNTR loci for better resolution and tracing of such strains, using a collection of 535 Beijing isolates from six world regions where these strains are known to be prevalent. The typeability and interlaboratory reproducibility of these hypervariable loci were lower than those of the 24 standard loci. Three loci (2163a, 3155, and 3336) were excluded because of their redundant variability and/or more frequent noninterpretable results compared to the 4 other markers. The use of the remaining 4-locus set (1982, 3232, 3820, and 4120) increased the number of types by 52% (from 223 to 340) and reduced the clustering rate from 58.3 to 36.6%, when combined with the use of the standard 24-locus set. Known major clonal complexes/ 24-locus-based clusters were all subdivided, although the degree of subdivision varied depending on the complex. Only five single- locus variations were detected among the hypervariable loci of an additional panel of 92 isolates, representing 15 years of clonal spread of a single Beijing strain in a geographically restricted setting. On this calibrated basis, we propose this 4-locus set as a consensus for subtyping Beijing clonal complexes and clusters, after standard typing.Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3911419/bin/supp_52_1_164__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3911419/bin/JCM.02519-13_zjm999093084so1.pdf
Authors & Co-Authors
Allix-Béguec, Caroline
France, Lille
Genoscreen
Wahl, Céline
France, Lille
Genoscreen
Hanekom, Madeleine
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Nikolayevskyy, Vladyslav N.
United Kingdom, London
Barts and the London School of Medicine and Dentistry
Drobniewski, Francis A.
United Kingdom, London
Barts and the London School of Medicine and Dentistry
United Kingdom, London
Imperial College London
Maeda, S.
Japan, Kiyose
The Research Institute of Tuberculosis, Japan Anti-tuberculosis Association
Campos-Herrero, Isolina
Spain, Las Palmas de Gran Canaria
Hospital de Gran Canaria Dr. Negrin
Mokrousov, Igor
Russian Federation, Saint Petersburg
Saint-petersburg Pasteur Institute
Niemann, Stefan
Germany, Borstel
Forschungszentrum Borstel - Zentrum Für Medizin Und Biowissenschaften
Kontsevaya, Irina
Russian Federation, Samara
Samara Oblast tb Service
Rastogi, Nalin
Guadeloupe, Pointe-a-pitre
Institut Pasteur de Guadeloupe
Samper Blasco, Sofía
Spain, Zaragoza
Hospital Miguel Servet
Spain, Madrid
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
Sng, Li Hwei
Singapore, Singapore City
Singapore General Hospital
Warren, Robin Mark
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Supply, Philip
France, Lille
Genoscreen
France, Paris
Inserm
France, Lille
Center for Infection and Immunity of Lille Ciil
France, Lille
Université de Lille
Statistics
Citations: 86
Authors: 15
Affiliations: 16
Identifiers
Doi:
10.1128/JCM.02519-13
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Infectious Diseases