Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Polymerase chain reaction-guided diagnosis of infective keratitis a hospital based study
Current Eye Research, Volume 37, No. 11, Year 2012
Notification
URL copied to clipboard!
Description
Purpose: To compare polymerase chain reaction (PCR) to microbial culture and smear for detection and identification of bacterial and fungal pathogens in suspected microbial keratitis. Materials and methods: Corneal scrapings from 88 patients with suspected infectious keratitis were subjected to routine bacterial culture and sensitivity, Gram's stain, fungal culture; potassium hydroxide (KOH) wet mount, and PCR. PCR was performed with primer pairs targeted to the 16S and 18S r RNA gene. The result of the PCR was compared with conventional culture and Gram staining method. Results: By broad-range PCR, 40 (45.45%) cases were positive for fungi (90.9% sensitivity), 26 (29.5%) were culture positive (59.09% sensitivity), 29 (33%) of all patients were positive for bacteria by broad-range PCR (87.9% sensitivity) and 19 (21.6%) were culture positive (57.58% sensitivity). The time taken for PCR assay was 48h whereas positive fungal cultures took 210 days and bacterial culture from 2 to 4 days. Smears were positive for fungi in 29 eyes (33% of cases, 65.91% sensitivity) and for bacteria in 11 eyes (12.5% of cases, 33.33% sensitivity). Conclusions: DNA amplification with universal primers is a promising diagnostic tool in cases of infectious keratitis where routine laboratory culture failed to identify the pathogen. PCR may be performed in cases where the results of corneal scraping stains are negative without waiting for the results of the culture. © 2012 Informa Healthcare USA, Inc.
Authors & Co-Authors
Abu El Einen, Khaled Gamal
Egypt, Cairo
Faculty of Medicine
Mohalhal, Ahmed A.
Egypt, Cairo
Faculty of Medicine
Elmekawy, Hany Elmekawy
Egypt, Cairo
Faculty of Medicine
Abdelbaki, Ahmed M.
Egypt, Cairo
Faculty of Medicine
Sherif, Ahmed Mohamed
Egypt, Cairo
Faculty of Medicine
El-Sherif, Rasha Hamed
Egypt, Cairo
Faculty of Medicine
Abdul Rahman, Eiman Mohammed
Egypt, Cairo
Faculty of Medicine
Statistics
Citations: 40
Authors: 7
Affiliations: 1
Identifiers
Doi:
10.3109/02713683.2012.698357
ISSN:
02713683
e-ISSN:
14602202
Research Areas
Genetics And Genomics
Health System And Policy