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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Genetic changes during laboratory propagation: Copy number At the reticulocyte-binding protein 1 locus of Plasmodium falciparum
Molecular and Biochemical Parasitology, Volume 172, No. 2, Year 2010
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Description
Comparative genomic hybridization studies have revealed elevated copy number (CN) at the reticulocyte-binding protein 1 gene (PfRh1) in fast growing lab-adapted parasites, while genetic manipulation demonstrates a causal link between cell invasion and PfRh1 CN. We therefore examined PfRh1 copy number variation (CNV) in 202 single clone parasite isolates from four countries to quantify the extent of CNV within natural populations. Surprisingly, we found that no natural parasite infections showed elevated CN. In contrast, 4/28 independent laboratory reference strains show elevated CN. One possibility is that amplification of PfRh1 (or neighboring loci) is selected during laboratory culture. In the case of FCR3 group of parasites, clone trees show that PfRh1 amplification arose in laboratory lines following establishment in culture. These data show that CNV at PfRh1 is rare or non-existent in natural populations, but can arise during laboratory propagation. We conclude that PfRh1 CNV is not an important determinant of gene expression, cell invasion or growth rate in natural parasite populations. © 2010 Elsevier B.V.
Authors & Co-Authors
Nair, Shalini K.
United States, San Antonio
Texas Biomedical Research Institute
Nkhoma, Standwell C.
United States, San Antonio
Texas Biomedical Research Institute
Nosten, François Henry
Thailand, Mae Sod
Shoklo Malaria Research Unit
United Kingdom, Oxford
Churchill Hospital
Thailand, Nakhon Pathom
Mahidol University
Mayxay, Mayfong
Democratic Republic Congo, Oxford
Mahosot Hospital
Democratic Republic Congo
University of Health Sciences
French, N.
Malawi, Chilumba
Malawi Epidemiology and Intervention Research Unit
Whitworth, James Ag
United Kingdom, London
Wellcome Trust
Anderson, Timothy J.C.
United States, San Antonio
Texas Biomedical Research Institute
Statistics
Citations: 18
Authors: 7
Affiliations: 8
Identifiers
Doi:
10.1016/j.molbiopara.2010.03.015
ISSN:
01666851
Research Areas
Genetics And Genomics