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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Evaluation of endometrial biomarkers for semi-invasive diagnosis of endometriosis
Fertility and Sterility, Volume 95, No. 4, Year 2011
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Description
Objective: To test the hypothesis that specific proteins and peptides are expressed differentially in eutopic endometrium of women with and without endometriosis and at specific stages of the disease (minimal, mild, moderate, or severe) during the secretory phase. Design: Patients with endometriosis were compared with controls. Setting: University hospital. Patient(s): A total of 29 patients during the secretory phase were selected for this study on the basis of cycle phase and presence or absence of endometriosis. Intervention(s): Endometriosis was confirmed laparoscopically and histologically in 19 patients with endometriosis of revised American Society for Reproductive Medicine stages (9 minimal-mild and 10 moderate-severe), and the presence of a normal pelvis was documented by laparoscopy in 10 controls. Main Outcome Measure(s): Protein expression of endometrium was evaluated with use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. The differential expression of protein mass peaks was analyzed with use of support vector machine algorithms and logistic regression models. Result(s): Data preprocessing resulted in differential expression of 73, 30, and 131 mass peaks between controls and patients with endometriosis (all stages), with minimal-mild endometriosis, and with moderate-severe endometriosis, respectively. Endometriosis was diagnosed with high sensitivity (89.5%) and specificity (90%) with use of five down-regulated mass peaks (1.949 kDa, 5.183 kDa, 8.650 kDa, 8.659 kDa, and 13.910 kDa) obtained after support vector machine ranking and logistic regression classification. With use of a similar analysis, minimal-mild endometriosis was diagnosed with four mass peaks (two up-regulated: 35.956 kDa and 90.675 kDa and two down-regulated: 1.924 kDa and 2.504 kDa) with maximal sensitivity (100%) and specificity (100%). The 90.675-kDa and 35.956-kDa mass peaks were identified as T-plastin and annexin V, respectively. Conclusion(s): Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry analysis of secretory phase endometrium combined with bioinformatics puts forward a prospective panel of potential biomarkers with sensitivity of 100% and specificity of 100% for the diagnosis of minimal to mild endometriosis. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.
Authors & Co-Authors
Kyama, Cleophas Mutinda
Belgium, Leuven
Ku Leuven
Belgium, Leuven
Ku Leuven– University Hospital Leuven
Kenya, Nairobi
National Museums of Kenya
Mihályi, Attila M.
Belgium, Leuven
Ku Leuven
Belgium, Leuven
Ku Leuven– University Hospital Leuven
Gevaert, A. Olivier
Unknown Affiliation
Waelkens, Etienne
Belgium, Leuven
Ku Leuven– University Hospital Leuven
Simsa, Péter
Belgium, Leuven
Ku Leuven
Belgium, Leuven
Ku Leuven– University Hospital Leuven
Van de Plas, Raf
Unknown Affiliation
Meuleman, Christel L.
Belgium, Leuven
Ku Leuven
de Moor, Bart L.R.
Unknown Affiliation
D'Hooghe, Thomas Maria
Belgium, Leuven
Ku Leuven
Kenya, Nairobi
National Museums of Kenya
Statistics
Citations: 38
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1016/j.fertnstert.2010.06.084
ISSN:
00150282
Research Areas
Health System And Policy
Sexual And Reproductive Health
Study Design
Randomised Control Trial
Cohort Study
Participants Gender
Female