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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
T lymphocytes among HIV-infected and -uninfected infants: CD4/CD8 ratio as a potential tool in diagnosis of infection in infants under the age of 2 years
Journal of Translational Medicine, Volume 3, Article 6, Year 2005
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Description
Background: Serologic tests for HIV infection in infants less than 18 months do not differentiate exposure and infection since maternally acquired IgG antibodies may be detected in infants. Thus, the gold standard for diagnosis of HIV-1 infection in infants under the age of 2 years is DNA or reverse transcriptase polymerase chain reaction. There is an urgent need to evaluate alternative and cost effective laboratory methods for early diagnosis of infant HIV-1 infection as well as identifying infected infants who may benefit from cotrimoxazole prophylaxis and/or initiation of highly active antiretroviral therapy. Methods: Whole blood was collected in EDTA from 137 infants aged 0 to 18 months. DNA polymerase chain reaction was used as the reference standard for diagnosis of HIV-1 infection. T-cell subset profiles were determined by flow cytometry. Results: Seventy-six infants were DNA PCR positive while 61 were negative. The median CD4 counts of PCR negative infants were significantly higher than those of the PCR positive infants, p < 0.001. The median CD4/CD8 ratio and the %CD4 of the PCR positive infants were both significantly lower than those of the negative infants, p < 0.001. The CD4/CD8 ratio had a >98% sensitivity for diagnosis of HIV-1 infection and a specificity of >98%. Conclusion: The CD4/CD8 ratio appears useful in identifying HIV-infected infants. The development of lower cost and more robust flow cytometric methods that provide both CD4/CD8 ratio and %CD4 may be cost-effective for HIV-1 diagnosis and identification of infants for cotrimoxazole prophylaxis and/or highly active antiretroviral therapy. © 2005 Zijenah et al; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC549040/bin/1479-5876-3-6-S1.doc
Authors & Co-Authors
Zijenah, Lynn Sodai
Zimbabwe, Harare
University of Zimbabwe
Katzenstein, David A.
United States, Stanford
Stanford University School of Medicine
Nathoo, Kusum Jackison
Zimbabwe, Harare
University of Zimbabwe
Rusakaniko, Simbarashe
Zimbabwe, Harare
University of Zimbabwe
Tobaiwa, Ocean
Zimbabwe, Harare
University of Zimbabwe
Gwanzura, Christine
Zimbabwe, Harare
University of Zimbabwe
Bikoue, Arsène
United Kingdom, London
Ucl Medical School
Nhembe, Margaret
Zimbabwe, Harare
University of Zimbabwe
Matibe, Petronella
Zimbabwe, Harare
University of Zimbabwe
Janossy, George J.
United Kingdom, London
Ucl Medical School
Statistics
Citations: 42
Authors: 10
Affiliations: 3
Identifiers
Doi:
10.1186/1479-5876-3-6
ISSN:
14795876
e-ISSN:
14795876
Research Areas
Genetics And Genomics
Infectious Diseases
Maternal And Child Health