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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Expanded numbers of circulating myeloid dendritic cells in patent human filarial infection reflect lower CCR1 expression
Journal of Immunology, Volume 185, No. 10, Year 2010
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Description
APC dysfunction has been postulated to mediate some of the parasite-specific T cell unresponsiveness seen in patent filarial infection. We have shown that live microfilariae of Brugia malayi induce caspase-dependent apoptosis in human monocyte-derived dendritic cells (DCs) in vitro. This study addresses whether apoptosis observed in vitro extends to patent filarial infections in humans and is reflected in the number of circulating myeloid DCs (mDCs; CD11c-CD123lo) in peripheral blood of infected microfilaremic individuals. Utilizing flow cytometry to identify DC subpopulations (mDCs and plasmacytoid DCs [pDCs]) based on expression of CD11c and CD123, we found a significant increase in numbers of circulating mDCs (CD11c+CD123lo) in filariainfected individuals compared with uninfected controls from the same filaria-endemic region of Mali. Total numbers of pDCs, monocytes, and lymphocytes did not differ between the two groups. To investigate potential causes of differences in mDC numbers between the two groups, we assessed chemokine receptor expression on mDCs. Our data indicate that filaria-infected individuals had a lower percentage of circulating CCR1+ mDCs and a higher percentage of circulating CCR5+ mDCs and pDCs. Finally, live microfilariae of B. malayi were able to downregulate cell-surface expression of CCR1 on monocyte-derived DCs and diminish their calcium flux in response to stimulation by a CCR1 ligand. These findings suggest that microfilaria are capable of altering mDC migration through downregulation of expression of some chemokine receptors and their signaling functions. These observations have major implications for regulation of immune responses to these long-lived parasites. Copyright © 2010 by The American Association of Immunologists, Inc.
Authors & Co-Authors
Semnani, Roshanak Tolouei
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Mahapatra, Lily
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Dembéle, Benoit
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Konaté, Siaka
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Metenou, Simon
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Dolo, Housseini
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Coulibaly, Michel Emmanuel
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Soumaoro, Lamine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Coulibaly, Siaka Yamoussa
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Sanogo, Dramane
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Doumbia, Salif Seriba
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Diallo, Abdallah Amadou
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Traoré, Sékou Fantamady
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Klion, Amy D.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Nutman, B. Thomas
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Mahanty, Siddhartha
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Statistics
Citations: 28
Authors: 16
Affiliations: 2
Identifiers
Doi:
10.4049/jimmunol.1001605
ISSN:
00221767
e-ISSN:
15506606
Research Areas
Cancer
Study Locations
Mali