Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Molecular diagnosis of Schistosoma infections in urine samples of school children in Ghana
American Journal of Tropical Medicine and Hygiene, Volume 88, No. 6, Year 2013
Notification
URL copied to clipboard!
Description
Recent studies using an internal transcribed spacer (ITS)-based real-time polymerase chain reaction (PCR) for the detection of Schistosoma DNA in urine samples has shown high sensitivity and specificity when performed on controls and known microscopy-positive samples. In this study, using 730 urine samples collected from children in five primary schools from different communities in the Greater Accra region of Ghana, specific detection of Schistosoma DNA showed excellent sensitivity of 100% and 85.2% in urines with > 50 eggs/10 mL urine and ≤ 50 eggs/10 mL of urine, respectively. Additionally, Schistosoma-specific DNA was amplified in 102 of 673 samples in which Schistosoma eggs could not be detected with microscopy. Taking microscopy and/or PCR-positive samples as true positives, the negative predictive value calculated was 94.6-100% for each school sampled as compared with 54.3-95.7% using microscopy. This ITS-based real-time PCR proves to be a powerful tool in epidemiological surveys of schistosomiasis providing more precise and sensitive results than microscopy. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Aryeetey, Yvonne A.
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Essien-Baidoo, Samuel
Ghana, Cape Coast
University of Cape Coast Ghana
Larbi, Irene A.
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Ahmed, Kwaku
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Amoah, Abena Serwaa
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Obeng, Benedicta Baffoah
Ghana, Accra
Noguchi Memorial Institute for Medical Research
van Lieshout, Lisette
Netherlands, Leiden
Leids Universitair Medisch Centrum
Yazdanbakhsh, Maria M.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Boakye, Daniel Adjei
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Verweij, Jaco J.
Netherlands, Tilburg
St. Elisabeth Ziekenhuis
Statistics
Citations: 39
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.4269/ajtmh.12-0571
ISSN:
00029637
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Maternal And Child Health
Study Design
Cross Sectional Study
Study Locations
Ghana