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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Frequent silencing of fragile histidine triad gene (FHIT) in Burkitt's lymphoma is associated with aberrant hypermethylation
Genes Chromosomes and Cancer, Volume 41, No. 4, Year 2004
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Description
The fragile histidine triad (FHIT) gene, a potential tumor-suppressor gene, is frequently inactivated in multiple human cancers. However, the FHIT gene remains largely unexplored in Burkitt's lymphoma (BL). Hence, we assessed whether loss of FHIT expression occurs in BL, and, if so, what is the mechanism of such loss. Lack of protein expression was observed in 50% of BL cell lines. Methylation-specific polymerase chain reaction (MSP) showed that 45% of BL cell lines carried aberrantly methylated FHIT alleles. Sequencing of bisulfite-treated DNA confirmed these data and indicated a very high density of methylation in all methylated alleles. Real-time, quantitative reverse-transcription PCR analysis indicated that attenuation of full-length FHIT transcription was correlated with methylation. Sequencing of transcripts illustrated that aberrant transcription resulting in loss of FHIT exons occurred more commonly in BL containing unmethylated FHIT genes. However, such transcripts often coexisted with full-length FHIT transcripts. Not surprisingly, therefore, loss of FHIT protein in BL correlated with CpG island methylation, rather than with aberrant transcription. FHIT methylation also was detected in 31% (16 of 51) of the primary BLs examined, including 2 samples whose derived cell lines also manifested FHIT hypermethylation. Aberrant methylation can thus occur in vivo. In summary, this report provides evidence that epigenetic modification frequently results in loss of FHIT expression in BL. © 2004 Wiley-Liss, Inc.
Authors & Co-Authors
Hussain, Azhar R.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Gutiérrez, Marina Inés
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Saudi Arabia, Riyadh
Kfnccc and r
Timson, Georgina
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Siraj, Abdul K.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Deambrogi, Clara
Italy, Vercelli
Università Degli Studi Del Piemonte Orientale Amedeo Avogadro
Al-Rasheed, M.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Gaidano, Gianluca
Italy, Vercelli
Università Degli Studi Del Piemonte Orientale Amedeo Avogadro
Magrath, Ian T.
Belgium, Brussels
International Network for Cancer Treatment and Research Inctr
Bhatia, Kishor G.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Belgium, Brussels
International Network for Cancer Treatment and Research Inctr
Saudi Arabia, Riyadh
Kfnccc and r
Statistics
Citations: 9
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1002/gcc.20099
ISSN:
10452257
Research Areas
Cancer
Genetics And Genomics
Study Approach
Quantitative