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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin
Virology, Volume 402, No. 1, Year 2010
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Description
Griffithsin (GRFT), Cyanovirin-N (CV-N) and Scytovirin (SVN) are lectins that inhibit HIV-1 infection by binding to multiple mannose-rich glycans on the HIV-1 envelope glycoproteins (Env). Here we show that these lectins neutralize subtype C primary virus isolates in addition to Env-pseudotyped viruses obtained from plasma and cervical vaginal lavages. Among 15 subtype C pseudoviruses, the median IC50 values were 0.4, 1.8 and 20.1nM for GRFT, CV-N and SVN, respectively, similar to what was found for subtype B and A. Analysis of Env sequences suggested that concomitant lack of glycans at positions 234 and 295 resulted in natural resistance to these compounds, which was confirmed by site-directed mutagenesis. Furthermore, the binding sites for these lectins overlapped that of the 2G12 monoclonal antibody epitope, which is generally absent on subtype C Env. This data support further research on these lectins as potential microbicides in the context of HIV-1 subtype C infection. © 2010 Elsevier Inc.
Authors & Co-Authors
Alexandre, Kabamba Bankoledi
South Africa, Johannesburg
National Institute for Communicable Diseases
Gray, Elin Solomonovna
South Africa, Johannesburg
National Institute for Communicable Diseases
Lambson, Bronwen E.
South Africa, Johannesburg
National Institute for Communicable Diseases
Moore, Penny L.
South Africa, Johannesburg
National Institute for Communicable Diseases
Choge, Isaac A.
South Africa, Johannesburg
National Institute for Communicable Diseases
Mlisana, Koleka P.
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
Abdool Karim, Salim S.
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
McMahon, James B.
United States, Frederick
National Cancer Institute at Frederick
O'Keefe, Barry R.
United States, Frederick
National Cancer Institute at Frederick
Chikwamba, Rachel K.
South Africa, Pretoria
The Council for Scientific and Industrial Research
Morris, Lynn
South Africa, Johannesburg
National Institute for Communicable Diseases
Statistics
Citations: 92
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.1016/j.virol.2010.03.021
ISSN:
00426822
e-ISSN:
10960341
Research Areas
Infectious Diseases