Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Molecular surveillance of drug-resistant Plasmodium vivax using pvdhfr, pvdhps and pvmdr1 markers in Nouakchott, Mauritania
Journal of Antimicrobial Chemotherapy, Volume 67, No. 2, Article dkr464, Year 2012
Notification
URL copied to clipboard!
Description
Objectives: Plasmodium falciparum and Plasmodium vivax occur in Mauritania. Drug-resistant P. falciparum has been reported, but the drug-resistance status of P. vivax is unknown. The aims of the present study were to determine the prevalence of mutant pvdhfr, pvdhps and pvmdr1 genes and of pvmdr1 gene amplification in P. vivax isolates in Nouakchott, the capital city of Mauritania, and to establish a baseline for molecular surveillance of drug-resistant P. vivax in the country. Patients and methods: Between 2007 and 2009, 439 febrile patients were screened for malaria in Nouakchott. The sequences of pvdhfr, pvdhps and pvmdr1 markers in 110 P. vivax isolates were determined by direct sequencing of PCR products. The pvmdr1 gene copy number was determined by real-time PCR. Results: The majority of the isolates with a successful PCR amplification (76/86, 88%) were characterized to be of the wild-type pvdhfr genotype, while the remaining 10 isolates carried the S58R and S117N double mutations. All isolates had the wild-type pvdhps genotype SAKAV. For pvmdr1, 75 of 103 (73%) had the wild-type Y976, and 28 (27%) carried the mutant F976. Most (98%) carried the mutant L1076 codon. Of 105 isolates, 102 (97%) had one copy and 3 (3%) had two copies of the pvmdr1 gene. Conclusions: The prevalence of mutations associated with antifolate resistance is low in Mauritania. Further studies are required to determine the roles of pvmdr1 mutations and gene amplification in conferring drug resistance. These data will serve as a baseline for further monitoring of drug-resistant malaria. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Authors & Co-Authors
Mint Lekweiry, Khadijetou
Mauritania, Nouakchott
University of Nouakchott Université de Nouakchott
Morocco, Marakech
Université Cadi Ayyad
Morocco, Marakech
Faculté Des Sciences Semlalia
Ould Mohamed Salem Boukhary, Ali
Mauritania, Nouakchott
University of Nouakchott Université de Nouakchott
Gaillard, T.
France, Toulon
Hia Sainte-anne a Toulon
Wurtz, Nathalie
France, Marseille
Imtssa Institut de Médecine Tropicale du Service de Santé Des Armées
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Bogreau, Hervé
France, Marseille
Imtssa Institut de Médecine Tropicale du Service de Santé Des Armées
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Hafid, Jamaleddine
Morocco, Marakech
Université Cadi Ayyad
Trape, Jean François
France, Marseille
Ird Institut de Recherche Pour le Developpement
Bouchiba, Housem
France, Marseille
Imtssa Institut de Médecine Tropicale du Service de Santé Des Armées
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Salem, Mohamed Salem Ould Ahmedou
Mauritania, Nouakchott
University of Nouakchott Université de Nouakchott
Pradines, Bruno
France, Marseille
Imtssa Institut de Médecine Tropicale du Service de Santé Des Armées
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Rogier, Christophe
France, Marseille
Imtssa Institut de Médecine Tropicale du Service de Santé Des Armées
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Basco, Leonardo K.
France, Marseille
Imtssa Institut de Médecine Tropicale du Service de Santé Des Armées
France, Marseille
Ird Institut de Recherche Pour le Developpement
Briolant, Sébastien
France, Marseille
Imtssa Institut de Médecine Tropicale du Service de Santé Des Armées
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Statistics
Citations: 13
Authors: 13
Affiliations: 8
Identifiers
Doi:
10.1093/jac/dkr464
ISSN:
03057453
e-ISSN:
14602091
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Mauritania