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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Use of patient-specific Leptospira isolates in the diagnosis of leptospirosis employing microscopic agglutination testing (MAT)
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 105, No. 4, Year 2011
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Description
Given the protean manifestations of leptospirosis, adequate laboratory support for diagnosis is necessary. Traditionally, the gold standard is the microscopic agglutination test (MAT) using a panel of Leptospira isolates representing a broad range of serogroups and serovars. It has been proposed that screening with serovars circulating in a region would enhance test performance. We assessed the diagnostic usefulness of MAT using both regionally obtained clinical Leptospira isolates and the specific isolates recovered from the tested patients. Serum obtained from 41 acute febrile patients (obtained on average 7.2 days [SD ± 5.2] after onset of fever) was tested using a standard panel of 24 serovars along with regional isolates recovered from human and animal blood cultures from different regions in Egypt and a patient's own isolate, if available, to establish additional MAT panels. Serum samples tested by a standard 24 panel with a cut-off of >1:800 revealed five patients with positive serology. Only one patient had a positive result using a regional panel or patient's own culture developed MAT. However, the serovar with the highest titers did not match the cultured serovar. Region-specific MATs did not appear to be reliable in detection of infection or in identifying the infecting serovar. © 2010.
Authors & Co-Authors
Murray, Clinton K.
United States, San Antonio
San Antonio Military Medical Center, Texas
Gray, Michael R.
United States, San Antonio
U.s. Army Medical Command
Mende, Katrin
United States, San Antonio
San Antonio Military Medical Center, Texas
United States, Rockville
National Cancer Institute Nci
Parker, Tina M.
Egypt, Cairo
Namru3
Samir, Ahmed
Egypt, Cairo
Namru3
Egypt, Giza
Cairo University
Rahman, Bassem A.
Egypt, Cairo
Namru3
Habashy, Engy Emil
Egypt, Cairo
Namru3
Hospenthal, Duane R.
United States, San Antonio
San Antonio Military Medical Center, Texas
Pimentel, Guillermo
Egypt, Cairo
Namru3
Statistics
Citations: 35
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.trstmh.2010.12.004
ISSN:
00359203
Research Areas
Health System And Policy
Study Locations
Egypt