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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
HLA-DR binding prediction and experimental evaluation of T-cell epitopes of mycolyl transferase 85B (Ag85B), a major secreted antigen of Mycobacterium tuberculosis
Medical Principles and Practice, Volume 14, No. 3, Year 2005
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Description
Objective: To identify T-cell epitopes of Ag85B by analysis of its sequence for prediction to bind HLA-DR alleles and evaluate the predicted peptides for recognition by T cells in antigen-induced proliferation assays. Materials/ Subjects and Methods: The complete sequence of Ag85B was analyzed for HLA-DR binding prediction to 51 HLA-DR alleles by using a virtual matrix-based prediction program (ProPred). Synthetic peptides covering the sequence of mature Ag85B were also analyzed for binding to HLA-DR alleles, and evaluated for recognition in antigen-induced proliferation assays with Ag85B-specific T-cell lines established from the peripheral blood mononuclear cells of 10 HLA-DR-heterogeneous tuberculosis patients. Results: The ProPred analysis of the full-length Ag85B (325 aa), signal peptide (40 aa) and the mature protein (285 aa) predicted their binding to 100, 76 and 98% of the 51 HLA-DR alleles, respectively. The analysis of 31 synthetic peptides for binding to HLA-DR alleles showed that 4 of them could bind >50% HLA-DR alleles, and were considered promiscuous. Testing of Ag85B-specific T-cell lines with synthetic peptides showed that all of the T-cell lines responded to one or more peptides of Ag85B, and 9 of the 10 cell lines responded to one or more of the four peptides considered promiscuous for binding to HLA-DR alleles. Conclusion: The ProPred program was useful in predicting the HLA-DR alleles binding regions of Ag85B and identifying the promiscuous peptides recognized by T cells. Copyright © 2005 S. Karger AG.
Authors & Co-Authors
Mustafa, Abu Salim
Kuwait, Kuwait City
Kuwait University
Abal, Adnan T.
Kuwait, Kuwait City
Kuwait University
Kuwait, Safat
Chest Diseases Hospital Kuwait
Shaban, Fatema A.
Kuwait, Kuwait City
Kuwait University
El-Shamy, Abdel Salam Mahmoud
Kuwait, Safat
Chest Diseases Hospital Kuwait
Amoudy, Hanady A.
Kuwait, Kuwait City
Kuwait University
Statistics
Citations: 27
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1159/000084629
ISSN:
10117571