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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Prevalence of carbapenemase-encoding genes including New Delhi metallo-β-lactamase in Acinetobacter species, Algeria
International Journal of Infectious Diseases, Volume 17, No. 9, Year 2013
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Description
Background: Nosocomial infections caused by carbapenem-resistant Acinetobacter spp are a global health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of carbapenem resistance in Acinetobacter spp clinical isolates recovered from three different hospitals in western Algeria from 2008 to 2012. Methods: A total of 113 Acinetobacter spp isolates were identified by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Antimicrobial susceptibility testing was carried out, and minimum inhibitory concentrations (MICs) were determined by the dilution method on Mueller-Hinton agar for β-lactams, aminoglycosides, fluoroquinolones, and colistin. The characterization of β-lactamases was investigated by phenotypic tests for the detection of metallo-β-lactamases and oxacillinases. Resistance genes were screened for by quantitative PCR and sequenced when positive. Results: Among the 113 isolates, 80 (70.8%) were found to be resistant to imipenem with MICs ranging from 64 to 512μg/ml. The blaOXA-23-like gene was detected in 50% (40/80) of the isolates and the blaOXA-24-like gene was detected in 21.2% (17/80) of the isolates. In addition, the metallo-β-lactamase blaNDM-1-like was detected in five isolates (6.2%). Conclusions: This study represents the first description of autochthonous Acinetobacter spp producing metallo-β-lactamase blaNDM-1-like and oxacillinases blaOXA-23-like and blaOXA-24-like in western Algeria. © 2013 International Society for Infectious Diseases.
Authors & Co-Authors
Mesli, Esma
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Algeria, Tlemcen
Université Abou Bekr Belkaid Tlemcen
Berrazeg, Meryem
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Algeria, Tlemcen
Université Abou Bekr Belkaid Tlemcen
Drissi, Mourad
Algeria, Tlemcen
Université Abou Bekr Belkaid Tlemcen
Bekkhoucha, Souad Naïma
Algeria, Oran
Hôpital Universitaire
Rolain, Jean Marc
France, Marseille
Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes
Statistics
Citations: 66
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/j.ijid.2013.02.024
ISSN:
12019712
e-ISSN:
18783511
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Algeria