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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Selection, phenotyping and identification of acid and hydrogen peroxide producing bacteria from vaginal samples of Canadian and East African women
PLoS ONE, Volume 7, No. 7, Article e41217, Year 2012
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Description
The common but poorly understood condition known as bacterial vaginosis (BV) increases vulnerability to HIV infection and is associated with the absence of H2O2-producing Lactobacillus. Vaginal lactic acid bacteria (LAB) produce anti-HIV factors such as organic acids and hydrogen peroxide (H2O2), and may bind and inactivate HIV particles during scavenging of mannose. These factors define potential criteria for initial selection of candidate probiotics to block heterosexual transmission of HIV. Therefore, the primary goal of this study was to characterize acid production on mannose and H2O2 production in vaginal isolates from Canadian adolescents (192 isolates, 16 individuals) and commercial sex workers in Nairobi, Kenya (576 isolates, 96 individuals). Selection of isolates from H2O2-detecting media suggested an idiosyncratic individual-level profile and extensive phenotypic diversity, including the identification of a subset of "double-strong" acid- and H2O2-producers with phenotypes similar to well-characterized probiotic strains. Molecular fingerprinting of all isolates by capillary electrophoresis of 16S-23S rRNA interspacer amplicons was coupled with chaperonin-60 universal target (cpn60 UT) sequencing in a subset, tentatively identifying 96% of isolates although only 19% were sequenced. Most isolates belonged to Lactobacillus, Streptococcus, Bifidobacterium or Gardnerella, with a total of 37 species in 15 genera, as well as 5 potentially novel organisms, identified in this study. This sensitivity was likely enhanced by phenotype-based selection on two chromogenic media formulations. Identification of double-strong isolates may provide a rational basis for selection and further characterization of vaginal probiotics, with potential application as part of HIV prevention initiatives in western Canada and East Africa. © 2012 Schellenberg et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3402533/bin/pone.0041217.s001.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3402533/bin/pone.0041217.s002.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3402533/bin/pone.0041217.s003.pdf
Authors & Co-Authors
Schellenberg, John J.
Canada, Winnipeg
University of Manitoba
Dumonceaux, Tim J.
Canada, Saskatoon
Saskatoon Research and Development Centre
Hill, Janet E.
Canada, Saskatoon
University of Saskatchewan
Kimani, Joshua
Kenya, Nairobi
University of Nairobi
Jaoko, Walter G.
Kenya, Nairobi
University of Nairobi
Wachihi, Charles
Kenya, Nairobi
University of Nairobi
Mungai, Jane Njeri
Kenya, Nairobi
University of Nairobi
Lane, Margo A.
Canada, Winnipeg
University of Manitoba
Fowke, Keith Raymond
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi
Ball, Terry Blake
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi
Canada, Winnipeg
Canadian Science Centre for Human and Animal Health
Plummer, Francis Allan
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi
Canada, Winnipeg
Canadian Science Centre for Human and Animal Health
Statistics
Citations: 23
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0041217
e-ISSN:
19326203
Research Areas
Infectious Diseases
Study Locations
Multi-countries
Kenya
Participants Gender
Female