Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Patterns of coordinate down-regulation of ARE-containing transcripts following immune cell activation
Genomics, Volume 84, No. 6, Year 2004
Notification
URL copied to clipboard!
Description
We evaluated the expression of over 900 AU-rich element (ARE)-containing transcripts in primary human T lymphocytes following stimulation with anti-CD3 and anti-CD28 antibodies and found that approximately 48% of these transcripts were regulated following T cell activation. We identified approximately 145 ARE-containing transcripts that were rapidly induced and then rapidly disappeared within 1 h after activation. Another 250 ARE-containing transcripts expressed in resting T cells were rapidly turned off within 30 min after activation. The rates of transcript disappearance correlated well with rapid mRNA decay measured following transcriptional arrest with actinomycin D. We identified a subset of ARE-containing transcripts that were rapidly induced following T cell activation that were also induced following lipopolysaccharide stimulation of THP-1 monocytes, and these transcripts exhibited rapid decay in both cell types. Our results suggest that ARE-mediated mRNA decay plays an important role in the precisely coordinated down-regulation of gene expression following immune cell activation. © 2004 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Raghavan, Arvind
United States, Minneapolis
University of Minnesota Medical School
Dhalla, M.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Bakheet, Tala
Unknown Affiliation
Ogilvie, Rachel L.
United States, Minneapolis
University of Minnesota Medical School
Vlasova-St Louis, Irina A.
United States, Minneapolis
University of Minnesota Medical School
Khabar, Khalid S.A.
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
United States, Cleveland
Cleveland Clinic Foundation
Williams, Bryan R.G.
United States, Cleveland
Cleveland Clinic Foundation
Bohjanen, Paul R.
United States, Minneapolis
University of Minnesota Medical School
United States, Minneapolis
University of Minnesota Twin Cities
Statistics
Citations: 60
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1016/j.ygeno.2004.08.007
ISSN:
08887543
Research Areas
Genetics And Genomics