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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Classification of the Adenylation and Acyl-Transferase Activity of NRPS and PKS Systems Using Ensembles of Substrate Specific Hidden Markov Models
PLoS ONE, Volume 8, No. 4, Article e62136, Year 2013
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Description
There is a growing interest in the Non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) of microbes, fungi and plants because they can produce bioactive peptides such as antibiotics. The ability to identify the substrate specificity of the enzyme's adenylation (A) and acyl-transferase (AT) domains is essential to rationally deduce or engineer new products. We here report on a Hidden Markov Model (HMM)-based ensemble method to predict the substrate specificity at high quality. We collected a new reference set of experimentally validated sequences. An initial classification based on alignment and Neighbor Joining was performed in line with most of the previously published prediction methods. We then created and tested single substrate specific HMMs and found that their use improved the correct identification significantly for A as well as for AT domains. A major advantage of the use of HMMs is that it abolishes the dependency on multiple sequence alignment and residue selection that is hampering the alignment-based clustering methods. Using our models we obtained a high prediction quality for the substrate specificity of the A domains similar to two recently published tools that make use of HMMs or Support Vector Machines (NRPSsp and NRPS predictor2, respectively). Moreover, replacement of the single substrate specific HMMs by ensembles of models caused a clear increase in prediction quality. We argue that the superiority of the ensemble over the single model is caused by the way substrate specificity evolves for the studied systems. It is likely that this also holds true for other protein domains. The ensemble predictor has been implemented in a simple web-based tool that is available at http://www.cmbi.ru.nl/NRPS-PKS-substrate-predictor/. © 2013 Khayatt et al.
Authors & Co-Authors
Khayatt, Barzan I.
Netherlands, Nijmegen
Radboud Institute for Molecular Life Sciences
Iraq, Sulaymaniyah
University of Sulaimani
Overmars, Lex
Netherlands, Nijmegen
Radboud Institute for Molecular Life Sciences
Netherlands, Nijmegen
Netherlands Bioinformatics Center
Siezen, Roland J.
Netherlands, Nijmegen
Radboud Institute for Molecular Life Sciences
Netherlands, Nijmegen
Netherlands Bioinformatics Center
Netherlands, Delft
Kluyver Center for Genomics of Industrial Fermentation
Netherlands, Wageningen
Ti Food and Nutrition
Francke, Christof
Netherlands, Nijmegen
Radboud Institute for Molecular Life Sciences
Netherlands, Nijmegen
Netherlands Bioinformatics Center
Netherlands, Delft
Kluyver Center for Genomics of Industrial Fermentation
Netherlands, Wageningen
Ti Food and Nutrition
Statistics
Citations: 72
Authors: 4
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0062136
e-ISSN:
19326203