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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Mapping the Peste des Petits Ruminants virus nucleoprotein: Identification of two domains involved in protein self-association
Virus Research, Volume 131, No. 1, Year 2008
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Description
For Mononegavirales, the template for transcription and replication is not the naked RNA but the nucleoprotein (N) encapsidated genomic and anti-genomic RNA. Because of this central role in the replication of these viruses, N has been the subject of numerous structural and functional mapping studies. Here, we report on the cloning of the Peste des Petits Ruminants virus (PPRV) N gene into the baculovirus vector and the expression of the protein in insect cells. By electron microscopy observation, we have shown that this recombinant PPRV N forms nucleocapsid-like particles in insect cells in the absence of other PPRV proteins, as reported for other paramyxoviruses. As it is known that the formation of these particles is first linked to the self-assembly of N, we have made several deletions in the PPRV N gene and expressed these mutants in insect cells. Analysis of these proteins by immunoprecipitation and electron microscopy observation enabled us to map the N-N interaction domains into two regions of PPRV N: aa 1-120 and 146-241. The fragment aa 121-145, which is not conserved within the morbillivirus group, is also required for the formation/stability of the nucleocapsid helical structure. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Bodjo, Sanne Charles
Austria, Vienna
International Atomic Energy Agency, Vienna
Lelenta, Mamadou
Austria, Vienna
International Atomic Energy Agency, Vienna
Couacy-Hymann, Emmanuel
Cote D'ivoire
Laboratoire Central de Pathologie Animale
Kwiatek, Olivier
France, Paris
Cirad
Albina, Emmanuel
France, Paris
Cirad
Gargani, D.
France, Paris
Cirad
Libeau, Geneviève
France, Paris
Cirad
Diallo, Adama
Austria, Vienna
International Atomic Energy Agency, Vienna
Statistics
Citations: 14
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1016/j.virusres.2007.08.005
ISSN:
01681702
Research Areas
Genetics And Genomics