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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Covalent labelling of histones with aurothiomalate: Gold‐labelled H2A‐H2B dimers assemble to octamers, which form crystalline helical tubes
European Journal of Biochemistry, Volume 161, No. 1, Year 1986
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Description
Histone octamers were covalently labelled with aurothiomalate at amino groups by the method of carbodiimide activation. The labelling procedure was demonstrated to result in the specific covalent coupling through a single bond of the heavy metal atom label to protein amino groups. Such octamers were dissociated to yield soluble H2A‐H2B dimers containing three gold atoms per dimer. The dimers were reconstituted with native H3‐H4 tetramers to form labelled octamers, which were crystallized to form helical tubes. This strongly suggests that this procedure resulted in minimal changes of protein conformation. Copyright © 1986, Wiley Blackwell. All rights reserved
Authors & Co-Authors
Greyling, H. Johann
South Africa, Cape Town
University of Cape Town
Hapgood, Janet Patricia
South Africa, Cape Town
University of Cape Town
Sewell, Trevor Trevor
South Africa, Cape Town
University of Cape Town
von Holt, Claus
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 4
Authors: 4
Affiliations: 1
Identifiers
Doi:
10.1111/j.1432-1033.1986.tb10133.x
ISSN:
00142956
e-ISSN:
14321033