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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Identification of a new phospholipase D in Carica papaya latex
Gene, Volume 499, No. 2, Year 2012
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Description
Phospholipase D (PLD) is a lipolytic enzyme involved in signal transduction, vesicle trafficking and membrane metabolism. It catalyzes the hydrolysis and transphosphatidylation of glycerophospholipids at the terminal phosphodiester bond. The presence of a PLD in the latex of Carica papaya (CpPLD1) was demonstrated by transphosphatidylation of phosphatidylcholine (PtdCho) in the presence of 2% ethanol. Although the protein could not be purified to homogeneity due to its presence in high molecular mass aggregates, a protein band was separated by SDS-PAGE after SDS/chloroform-methanol/TCA-acetone extraction of the latex insoluble fraction. This material was digested with trypsin and the amino acid sequences of the tryptic peptides were determined by micro-LC/ESI/MS/MS. These sequences were used to identify a partial cDNA (723. bp) from expressed sequence tags (ESTs) of C. papaya. Based upon EST sequences, a full-length gene was identified in the genome of C. papaya, with an open reading frame of 2424. bp encoding a protein of 808 amino acid residues, with a theoretical molecular mass of 92.05. kDa. From sequence analysis, CpPLD1 was identified as a PLD belonging to the plant phosphatidylcholine phosphatidohydrolase family. © 2012 Elsevier B.V.
Authors & Co-Authors
Abdelkafi, Slim
France, Marseille
Aix Marseille Université
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Abousalham, Abdelkarim
France, Villeurbanne
Institut de Chimie et Biochimie Moléculaire et Supramoléculaire
Fendri, Imen
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Ogata, Hiroyuki
France, Marseille
Laboratoire Information Génomique et Structurale
Barouh, Nathalie
France, Paris
Cirad
Fouquet, Benjamin
Unknown Affiliation
Scheirlinckx, Frantz
Unknown Affiliation
Villeneuve, Pierre
France, Paris
Cirad
Carrière, Frédéric
France, Marseille
Aix Marseille Université
Statistics
Citations: 14
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.gene.2012.03.033
ISSN:
03781119
e-ISSN:
18790038
Research Areas
Genetics And Genomics